腹透液对大鼠腹膜间皮细胞凋亡和增殖的影响

Effects of peritoneal dialysis solution on proliferation and apoptosis of rat peritoneal mesothelial cells

目的探讨腹透液对腹膜间皮细胞凋亡和增殖的影响。方法采用MTT比色法测定腹透液对体外培养的大鼠腹膜间皮细胞增殖的影响,同时应用流式细胞仪检测细胞周期和凋亡率。结果腹透液刺激1h后,体外培养的大鼠腹膜间皮细胞增殖抑制率、G0/G1期细胞比例、早期凋亡率均增高;腹透液中糖浓度越高,刺激时间越长,以上3个指标增高越明显。含4.25%葡萄糖的腹透液刺激3h后,细胞增殖抑制率达44.12%,G0/G1期细胞占71.95%,早期凋亡率达23.59%,远远高于不含糖的阴性对照组和含1.5%糖腹透液组(P<0.001),也高于4.25%甘露醇组(P<0.05)。结论高糖腹透液诱导体外培养的腹膜间皮细胞凋亡,抑制其增殖。

Objective To investigate the effects of peritoneal dialysis solution （PDS） on proliferation and apoptosis of rat peritoneal mesothelial cells （RPMCs）. Methods The proliferation of RPMCs treated with PDS containing glucose of different concentrations for different times in vitro were examined by MTT colorimeric assay. The cell cycles and cell apoptosis rate were determined by flow cytometry. Results After PDS treatment for 1 h, the cell proliferation inhibition rate, percentage of cells at G0/G1 stage and early cell apoptosis rate increased, and such increment was more manifest with higher glucose concentration in PDS and longer treatment time of the cells. After treatment with PDS containing 4.25% glucose for 3 h, the proliferation inhibition rate of the RPMCs reached 44.12%, the percentage of cells at the G0/G1 stage amounted to 71.95% and the early apoptosis rate reached 23.59%, which was several times higher than that of the negative control and 1.5% glucose/PDS groups, and also higher than that of 4.25% mannitol/PDS groups. Conclusion PDS containing high-concentration glucose can induce significant apoptosis and inhibit the proliferation of RPMCs in vitro.