Duffy抗原趋化因子受体参与人乳腺癌的淋巴结转移

Downregulation of Duffy antigen receptor for ehemokine (DARC ) is associated with lymph node metastasis in human breast cancer

目的探讨Duffy抗原趋化因子受体(DARC)与乳腺癌患者淋巴结转移的关系。方法乳腺癌原发灶组织标本75例,按淋巴结转移情况分为阳性组和阴性组,通过免疫组化技术检测DARC表达,并探讨其与乳腺癌淋巴结转移的关系。结果淋巴结转移阳性组中DARC强表达18例(48．6％),淋巴结转移阴性组中DARC强表达31例(81．6％),两组间差异有统计学意义(P< 0．01)。DARC强表达组与弱表达组的微血管密度(MVD)分别为(35．67±17．96)／HP和(53．38±20．29)／HP,差异有统计学意义(P<0．01)。此外,远处转移的13例患者中,有9例DARC弱表达(69．2％),4例DARC强表达(30．8％)。DARC表达与MVD、淋巴结转移状态及肿瘤远处转移呈负相关,而与患者的存活时间呈正相关。结论人原发乳腺癌组织中DARC表达降低和MVD增加可能易化肿瘤向淋巴结等远隔部位的转移。

Objective To analyze the relationship between Duffy antigen receptor for chemokines （DARC） and the metastasis potential in human breast cancer. Methods Breast cancer tissue sections from 75 patients, grouped according to the local lymph node status were examined immunohistochemically for protein level of DARC. Microvessel density （MVD） was counted by endothelial cells immunostained using anti-CD34 antibody. Results Strong positive DARC immunostaining in lymph node negative and positive groups was detected in 31 cases （81.6%） and 18 cases （48.6%）, respectively （P 〈 0.01 ）. MVD was （35.67±17.96）/HP and （53.38±20.29）/HP in DARC strong positive and less positive cases （P 〈 0.01 ）. In those patients with lung, bone, hepatic distant metastasis （13 cases）, 9 cases （69.2%） were DARC less positive, 4 cases （30. 8% ） were DARC strong positive. The correlation coefficient was -0. 412 between DARC expression and MVD and the corresponding value was - 0. 346 between DARC expression and lymph node status and - 0. 333 between DARC expression and distant metastasis in breast cancer. Conclusion DARC may play a negative role in the process of neoangiogenesis, and probably has an association with the lymph node status.