PC细胞来源的致瘤生长因子在非小细胞肺癌患者血清中的表达与化疗敏感性相关分析

Correlation between the expression of PCDGF in serum and the chemotherapeutic sensitivity in NSCLC

目的观察PC细胞来源的致瘤生长因子(PCDGF)在非小细胞肺癌(NSCLC)患者和正常人血清中的表达差异,及其与化疗疗效的关系。方法44例晚期初治的NSCLC患者和30例健康成人静脉血,采用PCDGF单抗作为一抗,随机选择部分样本进行Western blot观察电泳印迹;全部标本进行ELISA检测,比较PCDGF在NSCLC患者及正常人血清中的表达差异,分析NSCLC患者血清中PCDGF的表达差异与化疗疗效的关系。结果Western blot结果显示,在NSCLC患者和正常人血清中存在PCDGF的表达,NSCLC患者表达高于正常人。ELISA检测结果显示,NSCLC患者血清PCDGF(P<0．01)表达显著高于健康成人;化疗抗拒者的PCDGF表达高于正常人(P<0．01),化疗敏感者的PCDGF表达显著高于正常人(P<0．01);化疗抗拒血清PCDGF表达高于化疗敏感者(P< 0．05)。结论PCDGF在NSCLC患者血清中的高表达可能是NSCLC侵袭转移和增殖活跃的指标,其表达强度可能与化疗疗效相关,提示可能对铂类为主的化疗方案耐药。

Objective To investigate the expression of PC cell-derived growth factor （PCDGF） in the serum of non-small cell lung cancer （NSCLC） patients and healthy adults, and it＇s correlation with chemotherapeutic sensitivity. Methods The venous blood samples of 44 advanced NSCLC patients and 30 healthy adults were collected, and PCDGF mono-antibody was used for detection in the experiment. A part of specimens were randomly selected for Western-blot, and all specimens were eventually examined by ELISA. Chemotherapeutic response of these patients was recorded in order to analyze the correlation between PCDGF expression level and chemotherapeutic sensitivity. Results Western blot results indicated that there was PCDGF expression both in NSCLC patients and healthy adults, and the expressing intensity of PCDGF in NSCLC patients was higher than that in healthy adults. The result of ELISA showed PCDGF expression in the patients whoever was chemoresistant or chemosensitive was significantly higher than that in healthy adults （P 〈 0.01 ）, However, in chemoresistant patients, it was significantly higher than that in chemosensitive with a borderline statistical difference（P 〈 0.05 ）. Conclusion PC cell-derived growth factor is found to be not only expressed in healthy adult but also in NSCLC patient at a high level in the serum, which may indicate metastasis and active proliferation in NSCLC. The intensity of PCDGF expression may be correlated with chemotherapy response and the high level expressing of PCDGF may indicate resistant to platlnum-based chemotherapeutic regimen.