兰州百合鳞茎发育及低温解除休眠过程中内源激素的变化

Changes of endogenous hormones in Lilium davidii var.unicolor bulbs during bulb development and storage at low temperature for dormancy release

以兰州百合为试材,研究了鳞茎发育过程中以及2、6、10℃条件下保湿贮藏101 d内母鳞茎与新鳞茎中内源激素的变化。结果表明:鳞茎发育过程中内源ABA含量以及母鳞茎的GA3与ZR含量增加,而内源IAA含量以及新鳞茎的GA3与ZR含量下降。低温贮藏期间,母鳞茎与新鳞茎的GA3、IAA含量均有升高过程,而ABA含量呈下降趋势;新鳞茎的ZR含量呈下降趋势,母鳞茎的ZR含量也有升高过程。低温处理初期的34 d内,内源激素变化最为显著。不同贮藏温度相比较,ABA含量差异不大,GA3含量随温度升高而下降。在富含淀粉的新鳞茎中,GA3和ABA表现出极显著的负相关关系,而在淀粉含量较低的母鳞茎中GA3和ABA无相关性。通径分析结果表明,母鳞茎与新鳞茎的物质代谢机制不同,母鳞茎的物质变化受内源GA3的调控,新鳞茎主要是ABA作用的结果。

Changes of endogenous hormones in mother scales and in daughter scales of bulbs in Lilium davidii var. unicolor were studied during bulb development and 101 days storage at 2, 6 and 10℃ in moist sawdust. The results indicated that ABA concentration both in mother and daughter scales and GA3 concentration, ZR concentration in mother scales increased during bulb development, but IAA concentration in all scales and GA3 concentration, ZR concentration in daughter scales declined. During the low temperature storage, GA3 and IAA level in different parts of bulb had the increase phase, but ABA content decreased, and ZR content in daughter scales declined, but which in mother scales had the increase as well as. The notable changes in content of endogenous hormones occurred in the earlier 34 d of storage. Compared among different storage temperature, ABA content had little difference, but GA3 content decreased with the rising of temperature. In daughter scales which having plentiful starch, a significantly negative correlation was observed between GA3 and ABA content. but had no any correlations in mother scales which had little starch. Path analysis of results showed that metabolism mechanism in mother scales and in daughter scales were different markedly during bulb development and storage at low temperature for dormancy release. Substances changes in mother scales were regulated by endogenous GA3, but mostly by endogenous ABA in daughter scales.