医用微生态制剂分离菌株对白假丝酵母菌芽管形成的抑制作用

Inhibition of Candida albicans germination by strains separated from probiotic products for medicinal purposes

目的:研究市售医用微生态制剂分离的单菌株对白假丝酵母菌芽管形成的影响．方法:分离市售益生菌产品单菌株10株,采用结晶紫芽管染色法,通过计算出芽率检测各益生菌分离菌株培养上清,活菌体和热灭活菌体对白假丝酵母菌的出芽抑制．结果:五种微生态制剂单菌的中性培养上清对白假丝酵母菌的芽管生成均具有显著的抑制作用(P<0．01);长双歧杆菌1,嗜酸乳杆菌及两株芽孢杆菌的活菌可以抑制出芽:热灭活菌体无法抑制白假丝酵母菌的出芽．结论:市售医用微生态制剂具备抑制白假丝酵母菌出芽的抑菌效果．

AIM： To study the effect of the strains separated from probiotic products for medicinal purposes on the germ tube formation of Candida albicans. METHODS： Ten single strains were isolated from the probiotic products bought from the market, respectively. Crystal violet-based germ tube assay was used and the inhibition efficiency of the culture supernatant, live bacteria and heat-killed bacteria from each probiotic products were evaluated by germination rate. RESULTS： Neutralized spend culture suspension of all the single strains separated from probiotic products suppressed the germination of Candida albicans significantly （all P 〈 0.01）. Live bacteria of Bifidobacterium longum 1, Lactobacillus acidophilus and Bacillus inhibited the germination while all the heat-killed bacteria didn＇t. CONCLUSION： Probiotic products for medicinal purposes can inhibit the growth of germ tube of Candida albicans.