牡丹皮化学成分的液相色谱-飞行时间串联质谱分析

Rapid identification of compounds in Cortex Moutan by liquid chromatography-tandem mass spectrometry

目的分析牡丹皮中的化学成分。方法应用优化的高效液相色谱-质谱联用方法对牡丹皮供试品进行分析。结果较好地分离了单萜苷、鞣质和酚酮类等38种化学成分。在负离子检测模式下,由电喷雾质谱得到各成分的准分子离子峰,再根据串联飞行时间质谱获得进一步的结构信息,推测出其中38种主要成分的可能结构。并对牡丹皮中主要化学成分的质谱裂解规律进行了分析。结论本方法能快速检测牡丹皮的化学成分。

Aim To analyze the chemical constituents of Cortex Moutan by liquid chromatography coupled with electrospray ionization mass spectrometry. Methods An on-line optimized HPLC-DAD/MS^2 technique was employed. Results In the negative ion detection mode, 38 components such as monoterpene glucosides, galloylglucoses and acetophenones were isolated. Among them, over thirty compounds were identified, including paeonol, paeonilflorin, oxypaeoniflorin, benzoylpaeoniflorin, benzoyloxypaeoniflorin, galloylpaeoniflorin, galloyloxypaeoniflorin, mundanpioside A, C, D, E, H, etc. by the high performance liquid chromatography with diode-array detection in parallel with eleetrospray ionization and quadrupole-time of flight tandem mass spectrometry （ HPLC-DAD/ESI-MS2 ）. Conclusion This method can be used to rapidly determine the constituents of Cortex Moutan.