血管内皮生长因子受体-1与子痫前期的相关性

The Relativity of Vascular Endothelium Growth Factor Receptor-1 and Preeclampsia

目的探讨子痫前期患者血清中可溶性血管内皮生长因子受体-1(sVEGFR-1)水平和胎盘组织中膜型血管内皮生长因子受体-1(膜型VEGFR-1)的蛋白表达与子痫前期发病的相关性。方法用ELISA法和免疫组化法检测10例轻度子痫前期,10例重度子痫前期和10例子痫患者以及10例正常对照血清中sVEGFR-1水平和胎盘组织中膜型VEGFR-1蛋白的表达。结果子痫前期各组血清中sVEGFR-1水平明显高于正常对照组(P<0.05),随子痫前期的病情加重血清中sVEGFR-1水平有上升趋势,轻度子痫前期<重度子痫前期<子痫期。膜型VEGFR-1在胎盘的表达主要位于绒毛滋养细胞胞膜,绒毛血管内皮细胞胞膜,羊膜上皮细胞胞膜上,间质有少量表达。子痫前期各组胎盘组织中膜型VEGFR-1蛋白表达均明显低于正常对照组(P<0.05),随病情加重有下降趋势,轻度子痫前期>重度子痫前期>子痫期。子痫前期sVEGFR-1/膜型VEGFR-1较对照组高,随病情加重有升高趋势,轻度子痫前期<重度子痫前期<子痫期。结论子痫前期发病可能与血清中sVEGFR-1水平、胎盘组织中膜型VEGFR-1表达有关。膜型VEGFR-1在胎盘的表达可能与子痫前期有关。

Objectives （1） To investigate the levels of the serum soluble endothelial growth factor receptor-1 in women with preeclampsia and compare the difference between the groups of the mild preeclampsia, severe preeclampsia and eclampsia. For more, to analyze the relativity between the levels of the serum soluble vascular growth factor receptor-1 and preeclampsia. （2） To detect the expression of the membrane-bound VEGFR-1 protein in the placenta tissue of the women with preeclampsia and compare the difference between the groups of the mild preeclampsia,severe preeclampsia and eclampsiao Then to analyze the relativity between the expression of the membrane-bound endothelial growth factor receptor-1 and preeclampsia. Methods （1） The serum levels of sVEGFR-1 in 10 women with mild preeclampsia, 10 women with severe preeclampsia, 10 women with eclampsia, 10 women without preeclampsia were detected by the quantitative sandwich enzyme immunoassay technique. （2） Immunohistochemistry and quantitative analysis were used to detect membrane-bound VEGFR-1 contents and distribution in the placenta tissue. Results （1） The results showed that the serum sVEGFR-1 levels of all preeclampsia groups were relatively higher than those of control group （P〈0. 05） and that there were significant differences between the groups of the mild preeclampsia, severe preeclampsia and eclampsia. （2） In placentas, the positive staining was detected predominantly in the membranes of villus syncytiotrophoblast cells, extravillous trophoblast cells, and villus endothelial cells. In fetal membranes, the positive staining was detected predominantly in amnionic endothelial cells. （3） The contents of membrane-bound VEGFR-1 in placenta of preeclampsia groups were relatively lower than that of control group. There were significant differences between membrane-bound VEGFR-1 contents of the mild preeclampsia,severe preeclampsia and eclampsia （P〈0. 05）. （4） The ratio of the sVEGFR-1 concentration in serum and the membrane-bound VEGFR-1 contents in placenta of preeclampsia groups （sVEGFR-1/membrane-bound VEGFR-1） was relatively higher than that of control group （P〈0. 05） and there were significant differences between the groups of the mild preeclampsia, severe preeclampsia and eclampsia （P〈0. 05）. Conclusions （2） The serum sVEGFR-1 levels of preeclampsia groups were relatively higher than those of control group, the VEGFR-1 may be related to preeclampsia. （2） The contents of membrane- bound VEGFR-1 in placenta of preeclampsia groups were relatively lower than that of control group, the membrane-bound VEGFR-1 may relate to preeclampsia. （3） The ratio of the sVEGFR-1 concentration in serum and the membrane-bound VEGFR-1 contents in placenta of preeclampsia groups （ sVEGFR-1/membrane- boundVEGFR-1） was relatively higher than that of control group, the preeclampsia may contribute to VEGFR-1/ PLGF and sVEGFR-1/membrane-bound.