摘要
目的探讨外源性碱性成纤维细胞生长因子(bFGF)对新生大鼠缺氧缺血性脑损伤(HIBD)后海马胶质纤维酸性蛋白(GFAP)表达的影响。方法通过结扎并剪断7日龄新生Wistar大鼠右侧颈总动脉,吸入8%氧气和92%氮气2 h制备新生鼠HIBD模型,设假手术组、生理盐水对照组、bFGF治疗组。通过免疫组织化学方法和计算机图像分析技术检测3组大鼠不同时点(术后d4、7、10、17、24)海马CA1区GFAP表达强度变化。结果假手术组海马内GFAP阳性细胞数量和染色强度术后d7达高峰;对照组、治疗组GFAP表达较假手术组增多,治疗组增多更明显,术后d10达高峰,GFAP阳性细胞主要分布于海马CA1、CA3区,术后d4、10、17组比较差异有显著性(P均<0.05)。结论1.新生鼠HIBD后脑缺血易损伤区GFAP表达增加,可能与脑损伤后神经细胞再生有关;2.外源性给予bFGF可增强新生鼠脑缺氧缺血后中枢神经系统GFAP表达,在神经细胞损伤的修复中发挥一定保护作用。
Objective To investigate the influence of exogenous basic fibroblast growth factor （bFGF） on glial fibrillary acidic protein （GFAP） expression in neonatal rats with hypoxic - ischemic brain damage （HIBD）. Methods Eighty HIBD models of neonatal Wistar rats were made by shearing right arteria carotis communis and then breathing 8% oxygen and 92% nitrogen for 2 hours. The models were divided into 2 groups randomly： trial group of bFGF and control group of normal sodium. The other 40 rats were taken into the sham operation group. Expressions of GFAP were examined with immunohistochernical staining and image quantitative analysis at the 4th, 7th, 10th, 17th and 24th days after operation. Resdts The expression of GFAP in hippocarnpal CAI region of rats in sham operation group reached peak at the 7th day after operation. The expression of GFAP in control group increased and reached peak at the 10th day after ope ration, which GFAP - positive cells mainly appeared in hipgr.ampal CAI region and CA3 region. The expression of GFAP in hipgr.ampal CAI region of rats of trial group was higher than those of sham operation group and control group, which reached peak at the 10th day after the operation, there was significant difference in 3 groups at the 4th, 10th and 17th days after operation （all P 〈 0.05）. Conclusions 1. The expression of GFAP increased in hippocampal CAI region of neonatal rats with HIBD, and it may be involved in regeneration of neurocytes after HIBD. 2. Exogenous bFGF can enhance the expression of GFAP, which may play an important role in restoration of brain damage due to hvpoxia- isehemia.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2006年第18期1212-1213,1222,共3页
Journal of Applied Clinical Pediatrics
基金
贵州省优秀科技教育人才省长专项资金项目资助(2005-83)
