儿童幽门螺杆菌空泡毒素基因的研究

The research of Helicobacter pylori vacA genotypes in children.

目的探讨儿童幽门螺杆菌vacA基因型并分析各基因亚型与胃十二指肠疾病的关系。方法对80例H·pylori感染的儿童,采用PCR法扩增胃黏膜vacA基因亚型。结果南宁儿童H·pylorivacA基因型有s1a/m1和s1a/m2两种组合,基因频率分别为3·75%、66·25%。vacA基因各亚型在慢性浅表性胃炎及消化性溃疡中的检出率差异无显著性(P>0·05)。结论s1a/m2为南宁地区儿童幽门螺杆菌的vacA优势基因型。部分患儿同时感染多株不同vacA基因型H·pylori。vacA基因各亚型不能作为南宁地区儿童H·pylori菌株毒力强弱的指标。

Objective To investigate the distribution of H · pylori vacA genetypes and their association with gastric duodenal diseases in children. Methods Eighty biopsy samples from patients with H ·pylori infection were studied. PCR analysis for vacA genotypes was performed by using DNA extracted from biopsy specimens. Results In 80 cases, there were two vacA mosaicisms including 3 for sl a/ml （3.75 % ） and 55 for s l a/m2 （66. 25% ）. No statistical differences were found in the distribution of vacA genotype of H·pylori strain infection between chronic superficial gastritis and peptic ulcer （ P 〉 0. 05 ）. Conclusion In Nanning, vacAsl a/m2 genotype of H· pylori is predominant in children. Part of patients may be coinfected with different vacA genotypes ofH·pylori. The vacA genotype can not be considered the markers of H· pylori which is more pathogenic in children in Nanning.