神经生长因子对腺样囊性癌细胞系增殖与凋亡的影响

Influence of Nerve Growth Factor on the Proliferation and Apoptosis of Adenoid Cystic Carcinoma Cell Line

目的:研究神经生长因子(nerve growth factor,NGF)对腺样囊性癌细胞系增殖和凋亡的影响,为进一步揭示腺样囊性癌嗜神经侵袭以及沿神经播散的机理提供理论依据。方法:将人腺样囊性癌细胞系(ACC-2)和作为对照的人舌癌细胞系(Tca-8113)分别与不同浓度的NGF共培养24、48和72h。用MTT法及Annexin V-FITC/PI双标记流式细胞术,研究不同浓度人重组神经生长因子β(hβNGF),对人腺样囊性癌细胞系ACC-2及对照组舌癌细胞系Tca-8113增殖与凋亡的影响。结果:40 ng/mL和80 ng/mL的NGF对ACC-2细胞作用48 h和72 h后,ACC-2细胞的增殖水平明显高于其他各组,而NGF对ACC-2细胞的凋亡则没有影响;NGF对Tca-8113的增殖和凋亡均没有作用。结论:NGF作用于腺样囊性癌细胞,能有效促进腺样囊性癌细胞的增殖,这可能是腺样囊性癌嗜神经侵袭和沿神经播散的理论基础。

Objective： To investigate the influence of nerve growth factor （NGF） on proliferation and apoptosis of adenoid cystic carcinoma （ACC）, and the mechanism of perineural invasion and extension. Methods： ACC-2 cell line was targeted and Tca-8113 cell line was used as control. Both of them were co-cultured with different concentrations of NGF for 24, 48 and 72 hours. MTT was used to detect the proliferation of ACC-2 and Tca-8113. Annexin V-FITC/PI double staining was used to detect the apoptosis of tumor ceils. Results were studied by double labelling flow cytometer. Results： The proliferation of ACC-2 cells incubated with NGF in 40 ng/mL and 80 ng/mL for 48 and 72 hours was significantly higher than that of other groups. NGF had no effects on the apoptosis of ACC-2 cells. Proliferation or apoptosis of Tca-8113 cells were not effected by NGF. Conclusions： NGF can effectively promote the proliferation of ACC cells. It may be the theoretic base of perineural invasion and extension of ACCs.