脱硅细菌B．mucilaginosus Lv_(1-2)的培养条件研究

The Cultural Conditions of the Desilication Strain B.mucilaginosus Lv_(1-2)

在倡导环保和节能的现代工业中,生物选矿以能耗低,工艺简单,环境污染少等优点日益受到人们的关注。选矿茵种的筛选和培养是生物选矿中至关重要的一个环节。为了掌握细菌的生长特性,提高生物选矿的效率,本文对作者筛选到的脱硅细茵B．mucilaginosusLv_(1-2)的生长和培养条件进行了研究。实验结果表明,该茵的生长速度快,接种后约36 h进入生长对数期,60h进入稳定期。最适培养条件是：温度30℃,起始pH值为7．5,摇床转速为220rpm。细茵的浓度随装液量的增加而降低。能利用多种碳源和氮源,最佳碳源是葡萄糖,氮源为硫酸铵。

In the modern industry, advocating environment protection and energy saving, biological beneficiation has been paid more attention with low energy, simple operation and less pollution. The screening and culture of the bacteria are most important step. In order to manage the growth characteristics of the bacteria and to enhance the beneficiation efficiency, the cultural conditions of B. mucilaginosusLv1-2 were studied in this paper. The results showed that the strain entered the logarithmic growth phase after inoculating 36h, and the stable phase, after 60h. The optimum cultural conditions are as follows： temperature, 30 ℃ ; initial pH value, 7.5; shaking speed of rotary shaker, 220rpm. The concentration of the strain decreases with the increasing of the culture media in the flask. The optimum carbon resourse and nitrogen resourse are glucose and （NH4）2SO4 respectively.