新鲜肺癌组织的DNA含量分析

Flow cytometric analysis for DNA levels in fresh tissues of lung carcinoma

目的：研究新鲜肺癌组织的DNA含量。方法：采用流式细胞术对30例新鲜肺癌组织和5例正常对照组组织制成的单细胞悬液进行了DNA含量分析。结果：肺癌组G0/G 1、S、G2/M各时相比率和细胞增殖指教以及DNA指数与对照组存在显著性差异(P＜0．01)。结论：肺病变组织细胞DNA的流式细胞术分析是判定肺部肿瘤恶性化的敏感指标。

Objective： To Study the DNA levels in fresh tissues of lung carcinoma. Methods： We analyzed the single- cell suspensions of 30 fresh lung carcinoma tissues and 5 normal tissues of control group to get their DNA levels by using flow cytometry. Results： There were statistical differences in the rates of G0/G1 ,S,and G2/M, proliferative indices and DNA indices between lung carcinoma group and control group（P 〈0.01）. Conclusion： Flow cytometric analysis for cellular DNA levels of lung tumour is a sensitive marker on examining malignant change of lung turnouts.