电化学发光法在检测孕产妇乙肝中的应用

Application of HBsAg detected by electrochemiluminescence assay in pregnant women

背景与目的：探讨电化学发光法(electrochemiluminescence ECL)在检测孕产妇乙肝病毒中的应用价值,从而正确指导乙肝孕产妇产后的喂养方式。方法：用ELISA法和ECL法平行测定孕产妇血清乙肝病毒血清标志物(HBV-M),并对ELISA法检测表面抗原为阴性而ECL法为低浓度的标本,用荧光定量PCR测定病毒DNA。结果：ECL法表面抗原的检出率(9．66%)明显高于ELISA法(6．54%),p＜0．05,ELISA法检测为阴性而ECL法为低浓度的标本,病毒DNA均为阳性。结论：ECL法测定HVV-M的敏感性比ELISA法高,可以更灵敏的检出低浓度的表面抗原,从而可以更好的指导孕产妇产后的喂养方式,但HBcAb阳性率过高。

Objective： To investigate the clinical value of HBV detection by the method of ECL in pregnant women and direct them how to feed their children. Methods： HBV markers in serum were detected by ELISA and ECL respectively. We used FQ- PCR to detect HBV - DNA in those samples whose HBsAg were negative by ELISA, but were a little higher than that of normal women by ECL. Results： The rate of positive HBsAg by ECL and ELISA are 9.66%, 6.54% respectively. The difference between them is significant（p 〈 0.05）. HBV - DNA is positive in those samples whose HBsAg are a little higher by the method of ECL. Conclusion： The sensitivity of HBV markers detection by ECL is higher than ELISA, so we can direct pregnant women more correctly. Unfortunately, the positive rate of HBcAb by ECL is much higher.