肾康宁对肾小球肾炎治疗作用的实验及临床研究

Study on the Effect of Shenkangning on Glomerulonephritis

目的:观察中药肾康宁对家兔实验性肾炎黏附分子及细胞因子表达的影响,以及临床肾小球肾炎治疗前后尿蛋白的变化。方法:检测牛血清白蛋白所致急性实验性肾炎动物肾组织ICAM-1表达、血清IL-6水平的变化,以及临床肾小球肾炎肾康宁治疗前后血清IL-6水平、尿蛋白的变化。结果:肾康宁能明显降低实验动物血清IL-6含量,降低急性实验性肾炎模型中ICAM-1阳性细胞表达率,同时减轻肾小球基底膜(GBM)增厚及炎症细胞浸润,阻止毛细血管内微血栓的形成;在临床肾小球肾炎中,肾康宁能减少肾小球肾炎患者血清IL-6水平及24h尿蛋白含量。结论:肾康宁通过降低细胞因子IL-6水平和黏附分子ICAM-1的表达,从而抑制免疫细胞的趋化、浸润、活化和增殖、减少免疫复合物的沉积,使肾小球炎症反应减轻,有效缓解家兔急性实验性和临床肾小球肾炎,使肾功能得以改善。

Objective.To investigate the protective effect of Shenkangning on rabbit experimental nephritis and human glomerulonephritis. Shenkangning is a preparation composed of Chinese medicines, Methods： Active serological nephritis was induced by injection of BSA in rabbits. ICAM - 1 expression was examined in renal tissue of rabbits models. Serum IL- 6 and urinary protein excretion were measured in patients with glomerulonephritis. Results： Shenkangning had an significant inhibitory effect on the excretion of urinary protein. Shenkangning reduced the expression of ICAM - 1 in the kidney of rabbit with nephritis. Thickening of glomerular bases membrane（GBM） and infiltration of inflammatory cells and formation of micro thrombus in the capillary of kidney tissue were reduced, Conclusion： Shenkangning inhibits chemotaxis, infiltration, activation and proliferation of immune cells and reduces auto-antibody production by down - regulation of ICAM - 1 and IL- 6 expression, Thereby Shenkangning reduces glomerular inflammation and improves renal function, suggesting its role in prevention and treatment of renal disease,