抑癌基因KLF-6和APC在结直肠癌组织中的表达及临床意义

Expressions and clinical implications of kruppel-like factor 6 and APC in human colorectal carcinoma

目的研究抑癌基因KLF-6和APC在结直肠癌中的表达规律及其临床意义。方法应用RT-PCR和免疫组织化学法对32例结直肠癌组织和正常黏膜组织中KLF-6和APC基因mRNA及蛋白的表达进行检测和分析。结果KLF-6与APC的mRNA阳性表达率在结直肠癌组织中分别为37．5%和34．3%,明显低于正常结直肠黏膜组织的96．9%和93．8%(P＜0．05)。KLF-6与APC的免疫组化染色阳性表达率在结直肠癌组织中分别为28．1%和25．0%,明显低于正常结直肠黏膜组织的81．3%和84．4%(P＜0．05)。KLF-6与APC在结直肠癌中的表达存在相关性(P＜0．05),并与肿瘤组织的分化程度、浸润深度、淋巴结转移及临床分期均有明显相关性(P＜0．05)。结论抑癌基因KLF-6和APC的低表达可能与结直肠癌的发生、发展、转移及预后有关,且两者存在相关性。

Objective To investigate the expressions and clinical implications of kruppel-like factor 6（KLF-6） and APC in human colorectal carcinoma. Methods The expressions of KLF-6 and APC in tumor and normal tissues from 32 patients with colorectal carcinoma were investigated by RT-PCR and immunohistochemical technique. Results The expression rates of KLF-6 and APC mRNA were 37. 5% and 34. 3% in tumor tissue, 96.9% and 93.8% respectively in normal tissues （both P 〈 0.05） . The expression rates of KLF-6 and APC protein were 28. 1% and 25.0% in colorectal carcinomas, 81.3% and 84. 43% respectively in normal tissues （both P 〈 0.05） . There was a significant correlation between the expressions of KLF-6 and APC in colorectal carcinomas （ P 〈 0.05）. The expressions of KLF-6 and APC were significantly correlated with tumor differentiation, depth of infiltration, lymph node metastasis and clinical stage （ P 〈 0. 05）. Conclusion Down-regulations of KLF-6 and APC might play an important role in the carcinogenesis, development, metastasis of human colorectal carcinoma.