氟尿嘧啶联合黄芪对小鼠胃癌组织氨基酸代谢的影响

Effect of 5-flurouracil in combination with astragalus membranaceus on amino acid metabolism in mice model of gastric carcinoma

目的研究氟尿嘧啶联合中药黄芪对3-甲基胆葸(MC)诱发的小鼠胃癌氨基酸代谢的影响规律。方法建立MC诱发小鼠胃癌的模型,分别设立氟尿嘧啶治疗组(A组)、氟尿嘧啶加黄芪治疗组(B组)、氟尿嘧啶加高剂量黄芪治疗组(C组)、胃癌对照组(D组)、假手术组(P组)和正常组(N组),对各组标本进行病理检测,并测定胃癌标本游离氨基酸含量。结果MC诱发3个月时小鼠胃癌发癌率为48．7%。P组胃组织游离氨基酸中缬氨酸、蛋氨酸、亮氨酸、精氨酸和胱氨酸含量高于正常组织(P＜0．05),且与D组无明显差异(P＞0．05)。A、B、C、D组中的丝氨酸含量高于N组(P＜0．05)；A、B组谷氨酸含量明显高于P、N组(P＜0．05),且A、B、C、D组的数值呈逐渐下降的趋势。C、D组胃癌组织中脯氨酸含量明显高于P、N组(P＜0．05),且A、B、C组的数值呈逐渐升高的趋势。讨论MC诱发的小鼠胃癌模型中丝氨酸、脯氨酸升高可反映其氨基酸代谢紊乱；氟尿嘧啶与黄芪联合应用,可减少胃癌组织中谷氨酸含量而抑制肿瘤生长。

Objective To study the effect of 5-flurouracil（5-FU） in combination with astragalus membranaceus（AM） on amino acid metabolism in mice model of gastric carcinoma induced by 3-methylcholanthrene（MC）. Methods Mice gastric carcinoma models were established by 3-methylcholanthrene induction and randomly divided into different groups, and received 5-FU treatment（group A）, 5-FU plus AM（group B）, 5-FU plus a high dose of AM（group C）, no treatment（group D） . Normal mice were used as control（group N）. Free amino acid in the tumor specimens were examined. Results The levels of free Valine, Methionine, Leucine, Arginine and cystine in the tumor specimens in group D were significantly higher than that in group N（ P 〈 0. 05） . The levels of free serine in group A, B, C, D were significantly higher than that in group N. The levels of free glutamic acid in group A, B were significantly higher than that in group N（ P 〈 0. 05）. The levels of free proline in group C, D were significandy higher than that in group P, N（ P 〈 0. 05） . Conclusions The increasing levels of free serine and proline in tumor specimens in gastric cancer mice model reveals metabolic disturbance of amino acid. 5-FU plus astragalus membranaceus can decrease the level of free glutamic acid in the mice models, and inhibit tumor growth.