电针对脑缺血大鼠碱性成纤维细胞生长因子和血管生成素及其受体表达的影响

Effect of electroacupuncture on bFGF、Ang-2 and Tie-2 expression in rats with cerebral ischemia

目的观察电针对急性脑缺血大鼠血管新生的影响。方法将30只雄性Wistar大鼠随机分成3组：正常组、模型组、电针组。复制急性大脑中动脉缺血模型,电针“百会”、“水沟”穴,采用免疫组织化学方法,检测碱性成纤维细胞生长因子和血管生成素2及其受体的表达。结果电针组梗死灶周围碱性成纤维细胞生长因子和血管生成素2表达增强,分别为(0．039±0．009)和(0．080±0．023),与正常组和模型组比较差异有统计学意义(P＜0．05)；而血管生成素2受体的表达虽增强,但与其他两组比较差异无统计学意义。结论电针可以增强急性脑缺血大鼠梗死灶周围碱性成纤维细胞生长因子和血管生成素2的表达,促进了梗死灶周围的血管新生。

Objective To observe the effect of electroacupuncture （EA） on the angiogenesis in rats with cerebral ischemia. Methods 30 male Wistar rats were randomly divided into 3 groups： normal group,model group and EA group. After the establishment of acute middle cerebral artery occlusion （MCAO） , the rats were treated with EA on DU20 and DU26. Then the expression of bFGF, Ang-2 and Tie-2 by immunohistoehemieal staining was observed. Results The expression of bFGF and Ang-2 around the infarction locus of EA group increased remarkably compared with normal group and model group and the increase of Tie-2 was not significant. Conclusion EA can increase the expression of bFGF and Ang-2 around the infarction locus and promote the angiogenesis in rats with cerebral isehemia.