紫外线照射充氧自血回输对梭曼中毒家兔氧自由基代谢的影响

The effects of ultraviolet blood irradiation and oxygenation on oxygen free radical metabolism in rabbits with soman intoxication

目的探讨紫外线照射充氧自血回输(UBIO)对家兔急性梭曼中毒后氧自由基代谢的影响。方法将100只家兔随机分为5组,即正常对照组、中毒组、常规治疗组、UBIO治疗组及复合治疗组,各组于14d后检测家兔血清中丙二醛(MDA)含量、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及过氧化氢酶(CAT)活性。结果与正常对照组相比,家兔梭曼中毒后,血清MDA含量及CAT活性明显升高(P＜0．01),SOD、GSH-Px活性明显降低(P＜0．05)。但经UBIO或复合治疗后,与中毒组血清MDA含量比较明显降低(P＜0．01),SOD、GSH-Px及CAT活性明显升高(P＜0．05)。结论家兔梭曼急性中毒后可伴有明显的氧自由基损伤,UBIO能明显提高机体抗氧化损伤能力,可以应用于梭曼急性中毒的治疗。

Objective To investigate the effects of ultraviolet blood irradiation and oxygenation（UBIO） on oxygen free radical metabolism（OFRM） in rabbits with acute soman intoxication. Methods One hundred rabbits were randomly divided into five groups ： a control group, a soman intoxication group （I） , a soman intoxication plus routine therapy group （TR）, a soman intoxication plus UBIO therapy group （UBIO） and a soman intoxication plus complex therapy group （CT）. All the rabbits were intervened accordingly. Then the concentrations of malondiadehyde （MDA） and the activities of superoxide dismutase （SOD）, glutathionperoxidase （GSH Px） and catalase （CAT） in serum were determined at 14 d after various treatments. Results Compared with the control group,the concentration of MDA and the activity of CAT in the I group were significantly increased（ P 〈0.01 ） , while the activities of SOD and GSH Px were obviously decreased（ P 〈 0.05 ）. After UBIO or complex therapy, the serum level of MDA was significantly decreased in comparison with that in the I group（ P 〈 0.01 ） , while the concentrations of SOD, GSH Px and CAT were enhanced（P 〈 0.05）. Conclusion UBIO therapy can improve antioxidation activity against the injury caused by free radicals and could be used to treat acute soman intoxication, which causes injury from increased oxygen free radical concentrations.