高压静电场照射雏鸡免疫器官的细胞免疫变化

Changes of cellular immunity in immune organs of chicken treated with high voltage electrostatic field

目的探讨不同性质高压静电场对雏鸡免疫器官细胞免疫变化的影响。方法用组织化学染色及细胞培养技术和MTT测定法对高压静电场照射雏鸡免疫器官的T细胞数量及其对ConA增殖功能的动态变化进行了检测。结果高压正静电场照射雏鸡免疫器官的T细胞数量及其增殖功能明显高于高压负静电场照射雏鸡和对照雏鸡;而高压负静电场照射雏鸡免疫器官的上述各项检测指标均不同程度的低于对照雏鸡。结论高压正静电场照射对雏鸡免疫器官的细胞免疫功能有促进作用,而高压负静电场照射可使雏鸡免疫器官的细胞免疫功能降低或减弱。

Objective To investigate the effects of high voltage electrostatic field （HVEF） of different properties on cellular immune changes in immune organs of the treated chickens. Methods Dynamic changes of the number and proliferative response to concanavalin A （ConA） of T cells in immune organs of chickens treated with HVEF were determined by histochemistry staining, cellular culture technique, and [3-（4,5-dimethylthiazol-2-yl）]-2,5-diphenyltetrazolium bromide （MTT） method. Results The number and proliferative function of T cells in immune organs of chickens treated with positive HVEF was significantly elevated compared with control group and negative HVEF group. By contrast, the indexes above-mentioned in immune organs of chicks treated with negative HVEF was decreased compared with control group. Condusion The positive HVEF promotes cellular immune function in immune organs of chickens, but the negative HVEF lowers cellular immune function of immune organs of treated chickens.