大花蕙兰的组织培养和次生代谢物质的调控

The Tissue Culture of Cymbidium Grandiflorum and Regulation of Secondary Metabolite

以茎尖、茎段、叶片、根尖作为外植体,研究了大花蕙兰组织培养,同时也对次生代谢物质的调控因素进行了探讨。结果表明,茎尖是最佳的外植体;培养基MS+6-BA1.0mg/L+NAA0.1mg/L对大花蕙兰愈伤组织和原球茎的诱导最好;培养基MS+6-BA1.0mg/L+NAA0.5mg/L对大花蕙兰原球茎的增殖较好;激素积累和温度都能增加次生代谢物质的分泌。

Studies were carried out on tissue culture of Cymbidium grandiflorum, using terminal buds, stem sections, leaf blades and terminal roots as explants. At the same time, the discussions were continued on the regulative factors of secondary metabolite. The results showed that the terminal buds were the best explants, MS＋6-BA1.0mg/L＋NAA0.1mg/L was good for induction of callus and protocorm and MS＋6-BA1.0mg/L ＋ NAA0.Smg/L was more beneficial to proliferation of protocorm. The accumulation of hormone and tempera： ture could increase the secretion of secondary metabolite.