不同海藻凝集素对鲤免疫活性物的诱导作用

The Induction of Immunity Substance in Cyprinus carpio by Different Marine Algae Lectins

以条斑紫菜(Porphyra yezoensis)和裙带菜(Undaria pinnatifida)的凝集素作为免疫增强剂,来研究它对鲤免疫活性的诱导作用.实验结果表明,投喂和灌喂凝集素后,鲤血清中凝集素、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、溶菌酶活性与对照组相比都有提高,其中投喂条斑紫菜凝集素组比投喂对照组依次提高3.0倍、0.8倍、3.9倍、1.0倍;投喂裙带菜凝集素组比投喂对照组依次提高1.0倍、0.4倍、3.0倍、0.7倍,灌喂裙带菜凝集素组比灌喂对照组依次提高3.0倍、0.3倍、1.8倍;灌喂条斑紫菜凝集素组比灌喂对照组依次提高3.0倍、0.7倍.由此可看出:两种凝集素都可以作为一种免疫添加剂激活鲤的免疫系统,对鲤的非特异性免疫功能具有明显的增强作用.其中投喂条斑紫菜的效果更好.

Lectins abstracted from Porphyra yezoensis and Undaria pinnatifida were used as immunity-stimulants to study the immune activity of Cyprinus carpio. The results show the agglutinative activity, SOD activity, CAT activity and lysozyme activity in serum of Cyprians carpio increased in contrast to that of control group. The activity of fed lectin from Porphyra yezoensis group was 3.0, 0. 8,3.9 and 1.0 times, respedctively, as much as that of control group. The activity of fed lectin from Undaria Pinnatifida group was 1.0, 0.4, 3.0 and 0.7 times, respectively, as much as that of control group. The activity of infusing lectin from Undaria pinnatifida group was 3.0,0. 3 and 1.8 times, respectively, as much as that of control group. The activity of infusing lectin from Porphyra yezoensis group was 3.0 and 0.7 times, respectively, as much as that of control group. These results show that two lectins can be used as a kind of immunity-stimulants to activate immunity system and strengthen the nonspecific immunity function of Cyprinus caarpio. The effect of fed lectin from Porphyra yezoensis is the the best.