摘要
采用聚丙烯酰胺凝胶垂直板电泳对76只洼地绵羊的苹果酸脱氢酶(MDH)的多态性进行检测和分析结果发现,MDH基因座2个等位基因MDHF和MDHS的频率分别为0.4868和0.5132,3种基因型FF、FS和SS的频率分别为0.1447、0.6842和0.1711。另在MDH同工酶染色方法上也做了新的尝试。
The polymorphism of malate dehydrogenase （MDH） was detected using polyacrylamide gel electrophoresis in wadi sheep, The results indicate that there are two alleles at the loci of MDH, the frequencies of MDHF and MDHS are 0. 4868, 0. 5132, respectively; The frequencies of genotypes of FF, FS and SS are 0. 1447,0. 6842 和 0. 1711, respectively. At the same time, we tried a new dyeing method to research MDH isozymes.
出处
《草食家畜》
2006年第3期5-7,共3页
Grass-Feeding Livestock
基金
国家自然基金国际合作项目(30310103007
30410103150)
江苏省教育厅基金项目(NK051039)
