免疫乳中特异性抗体对3种细菌所致小鼠腹泻的被动免疫保护作用

Protective Effects of IgG Antibodies From Immune Milk Against Mice Diarrhea Induced by 3 Bacterias of Enterobacteriaceae

目的研究抗肠道杆菌的免疫乳对小鼠腹泻的被动免疫保护作用。方法以9株人肠道病原菌制备的混合抗原对孕牛进行系统免疫，收集免疫乳，分组进行动物实验，观察灌喂免疫乳组和非免疫乳组小鼠的腹泻情况。结果免疫初乳中针对9种致病肠道杆菌的特异性IgG效价为211～212，为非免疫初乳的128～256倍；免疫常乳中针对9种致病肠道杆菌的特异性IgG效价为26～28，为非免疫常乳的16～64倍。灌喂免疫初乳及效价为1：64的免疫常乳组对肠产毒性大肠杆菌、鼠伤寒沙门菌、福氏志贺菌所致小鼠腹泻均有保护作用。结论免疫乳中特异性IgG抗体对3种细菌所致小鼠腹泻有被动免疫保护作用。

Objective To study the effect of antibodies from immune milk on mice diarrhea induced by 3 baeterias of enterobacteriaceae. Methods Three strains pathogenic E. coli, 3 strains Salmonella and 3 strains Shigella were selected as antigens to make the mixed vaccine, and gestation cows were immunized systemically. Immune milk with 9 milk antibodies was obtained. The test with the mice was done and the difference in mice diarrhea induced by 3 bacterias of enterobacteriaeeae feeded by immune milk or regular milk was observed. Results The antibody titer against 9 pathogenic bacteria in immune colostrums was 211-212,128-256 folds of that in the control regular colostrums. The antibody titer against 9 pathogenic bacteria in immune milk was 26-28, 16-64 folds of that in the control group of the ordinary milk. The test of the mice indicates that immune colostrums and immune milk of the antibody titer of 1：64 has function of passive immune protection for mice diarrhea induced by 3 bacterias of enterobacteriaceae. Conclusion Compared with the regular milk, the specific IgG isolated from immune milk can protect mice from diarrhea challenged with 3 bacteria of enterobacte.