魔芋微型试管芋(拟球茎)繁殖技术研究

Study on Progenitive Technology of Subminiature Cuvette-Tuber ofA konjac

在魔芋愈伤组织的基础上，研究了培养基组成、培养条件等因素对试管芋形成的影响。魔芋愈伤组织在添加6-BA3．0mg·L^-1、NAA0．5mg·L^-1、IBA0．5mg·L^-1、KT5．0mg·L^-1的MS培养基上，20d后形成大量不定芽。待不定芽生长至约0．5—1cm时，将温度控制在15—20℃之间，不定芽基部膨大，2个月后形成多达10个以上的微型种芋，种芋之间以愈伤组织相连。将微型种芋连同愈伤组织取出，栽培到适当的基质中，浇灌营养液使基质保持湿润，自然条件下萌发，出苗率在90％以上，叶片多达18片，当年每块微型种芋可形成5g以上的多个子芋。

The influence of culture medium and culture condition on A. konjac was studied. Adding 6 - BA3. 0mg · L^-1 ,NAA0.5mg· L^-1 , IBA0.5mg·L^-1 and KT5. 0mg·L^-1 on MS culture medium,a large number of buds could be produced on the callus after 20 days. When the buds stretched to 0.5 - 1.0cm, the temperature was controlled at 15 - 20℃. The base of buds expanded and formed subminiature cuvette - tuber after two months, each of cuvette - tubers was joined with callus. The subminiature cuvette - tuber and callus were planted into soil, the budding ratio was over 90%.