粉红聚端孢菌的生物学特性研究

Research on biological characteristics of Trichothecium roseum

在不同的温度、光照、酸碱度和营养等条件下对甜瓜主要采后致病菌——粉红聚端孢的菌丝生长和孢子萌发情况进行了研究．结果表明：病原菌菌丝生长温度范围为10～35℃，最适生长温度为25℃．病原菌菌丝在pH3～8的范围内均能生长．最适pH值为6．光照条件对菌丝的生长无显著影响．离体培养的营养试验中碳源以添加甜瓜汁液的培养基菌丝生长最好．其次为添加葡萄糖的培养基（PDA）；氮源则以添加VB的培养基菌丝生长效果最好．孢子在无菌蒸馏水中的萌发温度范围为10～35℃，最适温度为25℃，此时孢子萌发率最高．为50％．孢子萌发的pH为3．0～8．0，但在pH值为4．0～7．0时萌发率较高,结果还表明,分生孢子在黑暗条件下萌发速度快且萌发率高．

Effect of temperature,pH,light and nutrition condition on hyphal growth and spore germination of Trichothecium roseum as a postharvest pathogen of muskmelon were studied. The results were as follows：suitable temperature for hypha growth was 10～35 ℃, the optimum temperature for growth was 25℃. The hypha could grow under pH3～8, the optimum pH value was 6, and it was not sensitive to light. The hypha grew best on media with the juice of muskmelon as C-source and V B as N-source. In the sterile distilled water,the temperature for spore germination of Trichothecium roseum was 10～35℃, the optimum temperature was 25℃ ,and at this temperature,the germination rate reached the highest 50%. The pH3.0～9.0 was suitable for the spore germination of Trichothecium roseum, and the optimum pH value was 4.0～7.0. Darkness was benefit for the spore germination of Trichothecium roseum.