用活性碳分离纯化苯丙氨酸与酪氨酸的研究

Study on isolation and purification of L-Phenylalanin and L-Tyrosine by active carbon

根据活性炭对L-苯丙氨酸与L-酪氨酸的选择性吸附特性,通过活性炭吸附及氨水、乙醇溶液解吸,从啤酒酵母提取液中成功地分离提取了符合标准的L-苯丙氨酸与L-酪氨酸;实验研究了苯丙氨酸与酪氨酸在不同pH值溶液中在活性炭上的吸附平衡,确定了以pH值5.8上柱,1 mol氨水和60%乙醇二步洗脱分离提取两种氨基酸的工艺,为从啤酒酵母中提取氨基酸提供了一条有效的途径.

L-Phenylalanin （L-Phe） and L-Tyrosine （L-Tyr） were separated from the beer yeast extract using their selective adsorption on active carbon followed by elution with aqueous ammonia and ethanol. The obtained substances are in accordance with their corresponding product standards. The adsorption of L-Tyr and L-Phe on commercial activated carbon at different pH values in the solution has been investigated. An optimal separation technology is proposed with the adsorption carried out at the pH value of 5.8 followed by elution with 1mol/L aqueous ammonia and 60% ethanol, respectively. The method gives an effective separation of amino acids from the beer yeast extract.