酶法提取白灵菇深层发酵菌丝体多糖的研究

Enzymatic extraction of the submerged mycelium polysaccharide from Pleurotus nebrodensis

为提高白灵菇菌丝体多糖得率,采用二次回归正交旋转组合设计方法,对白灵菇液态深层发酵菌丝体多糖的酶法提取工艺条件中的酶用量、酶解温度、酶解时间、pH值4因子的最优化组合问题进行了研究,建立了具有良好预测性能的白灵菇菌丝体多糖提取条件模型,并利用回归模型结合M atlab软件对工艺条件的最优组合、各单因子效应以及双因素效应进行分析。结果表明,当酶用量为2.53%、酶解温度39.6℃、酶解时间3.2 h、pH值7.2时,多糖得率最高可达8.23%。

In order to improve the extraction rate of Submerged Mycelium Polysaccharide from Pleurotus nebrodensis, the influences of enzyme dosage , enzymolysis temperature, enzymolysis time and pH value on its yield were studied. A quadric regression model was established. The model fitted significantly well and the optimum combination was obtained. The responses of the polysaccharides yield to all four single factors and their double factors interaction were discussed. Results show that when the extraction parameters are controlled at enzyme dosage 2.53%, enzymolysis temperature 39.6℃, enzymolysis time 3.2 h and pH value 7.2, the polysaccharides yield could be up to 8.23%.