摘要
在成龄的菠萝蜜树茎干上取徒长的嫩茎为外植体,研究了菠萝蜜嫩茎离体培养的条件.结果表明:外植体芽萌发的最适培养基为MS+6-BA 2.0 mg.L-1+KT 1.0 mg.L-1+NAA 0.5mg.L-1;暗培养有助于芽的萌发;芽增殖的较适宜培养基为MS+6-BA 2.0 mg.L-1+KT 1.0mg.L-1+NAA 0.1 mg.L-1,连续培养7代后,其芽的平均增殖系数为4.3;本试验中增殖芽的诱导生根较难,还有待进一步地研究.
The explants were taken from the mature stem of Artocarpus heterophfllus in order to study the conditions of on off-body tissue culture of this plant. The following results were found ： 1 ） The best suitable medium for the explants germination is MS ＋ 6-BA2.0mg/L ＋ KT1.0mg/L ＋ NAA0.5mg/L; 2） darkness culture is conducive to the bud germinating; 3 ） The better suitable medium for the buds proliferation is MS ＋ 6-BA2.0mg/L ＋ KT1.0mg/L ＋ NAA0.1 mg/L;4） the average of the proliferation coefficient of the buds what have been cultured continuously for 7 generations is 4.3. There is room for research on the propagated buds farther.
出处
《海南大学学报（自然科学版）》
CAS
2006年第3期289-293,共5页
Natural Science Journal of Hainan University
关键词
菠萝蜜
离体培养
芽增殖
嫩茎
off-body tissue culture
bud proliferation
young stem
Artocarpus heterophyllus
