SO_2体内衍生物对人血红细胞的氧化损伤及其防护

Oxidation damage of sulfur dioxide derivatives on human red blood cells and the protection role of vitamin C

为了探讨二氧化硫(SO2)对哺乳类细胞的毒性作用及其防护.以SO2体内衍生物-亚硫酸盐和亚硫酸氢盐(分子比为3∶1)及抗坏血酸(维生素C,Vc)单独或同时处理人血红细胞,并测定红细胞过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性及脂质过氧化(LPO)水平.结果表明,SO2体内衍生物单独处理后,CAT和SOD活性均显著甚至极显著降低,而LPO水平均极显著升高.Vc单独处理后,CAT及SOD活性均有所升高,但差异不显著;而LPO水平除Vc为0.05mmol·L-1剂量组外,其余各组均极显著降低.0.1mmo·lL-1Vc和不同剂量SO2衍生物共同处理后,Vc使各组CAT、SOD活性均有升高,且Vc也降低了SO2衍生物引起的人血红细胞LPO水平.由此认为,SO2体内衍生物可以使人血红细胞中CAT和SOD的活性降低、LPO水平增高,Vc可起到一定防护作用.

To study the toxicological role of SO2 on mammalian cells and its protection. After treated by i. p. sulfur dioxide derivatives （sodium hydrosulfite and sodium sulfite 3 ： 1, mol·L^-1 / mol·L ^-1 or/and vitamin C, the activities of CAT （ catalase）, SOD （ superoxide dismutase） and the level of lipid peroxidation （LPO）in human red blood cells were investigated. Results showed that （1） After treated solely by sulfur dioxide derivatives, the activities of CAT and SOD were significantly decreased, the levels of LPO were increased significantly. （2） Vc caused increase of the activities of SOD and CAT, but not significant. And it caused significant decrease of the level of LPO except for the 0. 05 mmol·L^-1 vitamin C group. （3） After treated synchronously by Vc 0.1 mmol·L^- 1 and different dose derivatives of sulfur dioxide, Vc decreased level of LPO and increased the activities of CAT and SOD in the red blood cells treated by derivatives of sulfur dioxide. It is suggested that derivatives of sulfur dioxide might cause the decreases of CAT and SOD activities and the increases of LPO levels in human red blood ceils, Vc has protection role for it.