痕量氟的Al^（3＋）－Calcein配合物荧光熄灭测定法

DETERMINATION OF TRACE FLUORIDE WITH Al￣(3+)-CALCEIN COMPLEX BY FLUORO-QUENCHING METHOD

本文建立了痕量氟的Ａｌ３＋－Ｃａｌｃｅｉｎ配合物荧光熄灭测定法．该法激发波长４８０ｕｍ，发射波长５０３ｕｍ，线性范围２～１５０μｇ／Ｆ－，灵敏度为２μｇ／ＬＦ－，１００μｇ／ＬＦ－时标准偏差为１．３μｇ／ＬＦ－，是现有的最灵敏方法之一．用茜素络合剂分光光度法进行对照实验，二者获得的结果一致．

A fluoro-quenching method for the determination of fluoride within Al3+-Calcein complex is established. The excitation and emission wavelengths are 480um and 503nm, respectively. Rectilinear ranges is 2-150μg/L F-. The sensitivity of the method i3 2μg/L F- and the standard deviation of determination is 1. 3μg/L F -for 100μg/L of fluoride. The obtained results agree with those by spectrophotometry with alizarin complex. Theaccuracy and precision of the present method is satisfactory.