5＇端序列对hGM－CSF在大肠杆菌中表达水平的影响

Affects of hGM-CSF5' Sequence on Its Expression in E. coli

设计并构建了三种起始密码子ＡＴＧ上下游不同序列的人ＧＭ－ＣＳＦ原核表达质粒，对其核苷酸序列进行分析验证后，使它们分别在大肠杆菌中表达了人ＧＭ－ＣＳＦ．结果表明：ｐＬＧＭ３对ＧＭ－ＣＳＦ的表达量比ｐＬＧＭ１高约６倍，比ｐＬＧＭ２高约３倍．

It has designed and constructed human GM-CSF protokaryotic expression plasmids with three different upstream and downstream sequences of ATG original codon. After the three sequences have been determined, they were expressed in E. coli respectively. The results indicated that the production of GM-CSF in E. coli with PLGMI was about 6 times and 3 times higher than that of GM-CSF in E.coli with PLGM2, respectively.