摘要
目的探讨骨桥蛋白(osteopontin,OPN)和骨连接蛋白(osteonectin,ON)在乳腺癌组织中的表达及其与微钙化形成的关系。方法根据钼靶 X 线片中微钙化的有无及数目的多少(20枚),将93例乳腺癌分为元钙化组、少量钙化组、大量钙化组,用免疫组织化学方法检测各组OPN、ON 的表达水平及其与微钙化的关系。同时,选取3例含微钙化的乳腺癌组织行组织病理连续切片和透射电镜观察。结果组织切片显示:钙化不仅出现于坏死灶内,生长活跃的癌细胞巢内及周围组织同样可见颗粒状钙化;透射电镜观察显示:生长活跃的乳腺癌细胞胞质内可见深电子密度的钙盐颗粒;乳腺癌组织中 OPN、ON 高表达(79.6%、77.4%)且与钼靶片中微钙化的出现相关,随着钙化数目的增多,两者表达程度逐渐增强,钙化组与无钙化组比较差异有统计学意义(X^2值分别为11.454、5.540,P 值均<0.05);多钙化组与无钙化组比较,差异也具有统计学意义(X^2值分别为13.738、7.659,P 值均<0.05)。免疫组织化学染色显示钙化周围 OPN、ON 高表达。结论乳腺癌组织中 OPN、ON 高表达,且表达水平与微钙化密切相关。OPN、ON 可能参与了乳腺癌微钙化的形成过程。
Objective To investigate the expression of osteopontin （OPN） and osteonectin （ON） in breast cancer and its relationship with formation of microcalcification. Methods Acoording to the number of microcalcification of breast cancer in mammography, 93 cases were divided into three groups： nonmicrocalcification group, little microcalcification group, and much microcalcification group. The relationship between expression of OPN ／ ON and microcalcification was studied. At the same time, breast cancer with microcalcification was observed in 3 cases by using patho-histological method and transmission electron microscopy （TEM）. Results Microcalcification was detected not only in necrosis foci, but also in nest of breast cancer without necrosis. Microcalcification was found in intracytoplasm of breast cancer cells through TEM. Expression of OPN ／ ON was significant related with the presence and number of microcalcification. There was obviously different expression between microcalcification group and non-microcalcification group （χ^2 = 11. 454, 5.540, P 〈 0. 05）, and more obvious different expression between much microcalcification group and non-microcalcification group （χ^2 = 13. 738, 7.659,P 〈0.05）. OPN／ON showed high expression in tissue around the microcalcification. Conclusion Breast cancer tissue produces bone matrix protein OPN and ON, and they take part in the formation of microcalcification.
出处
《中华放射学杂志》
CAS
CSCD
北大核心
2006年第9期953-956,共4页
Chinese Journal of Radiology
