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STEM CELLS AND PROTEOMICS

STEM CELLS AND PROTEOMICS
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摘要 The distinctive features of proteomics are large-scale and high throughput. The key techniques of proteomics are two-dimensional gel electrophoresis, mass spectrometry and bioinformatics. Stem cell can differentiate into all kinds of cells, tissues and organs. There are many proteins and cytokines involved in the process of differentiation. Applying proteomics techniques to the research of the complex process of stem cell differentiation is of great importance to study the mechanism and applications of stem cell differentiation. The distinctive features of proteomics are large-scale and high throughput. The key techniques of proteomics are two-dimensional gel electrophoresis, mass spectrometry and bioinformatics. Stem cell can differentiate into all kinds of cells, tissues and organs. There are many proteins and cytokines involved in the process of differentiation. Applying proteomics techniques to the research of the complex process of stem cell differentiation is of great importance to study the mechanism and applications of stem cell differentiation.
机构地区 Stem Cell Center
出处 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2006年第3期161-167,共7页 中国癌症研究(英文版)
基金 This work was supported by the National Outstanding Youth Foundation of China (No. 30225038) and the National Key Research Project of China (No. 00CB510103).
关键词 PROTEOMICS Two-dimensional gel electrophoresis Mass spectrometry BIOINFORMATICS Stem cell Proteomics Two-dimensional gel electrophoresis Mass spectrometry Bioinformatics Stem cell
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  • 1Blackstock WP, Weir MP. Proteomics: quantitative and physical mapping of cellular proteins [J]. Trends Biotechnol 1999; 17:121-7.
  • 2Anderson NL, Anderson NG. Proteome and proteomics:new technologies, new concepts, and new words [J].Electrophoresis 1998; 19:1853-61.
  • 3Simpson R J, Dorow DS. Cancer proteomics: from signaling networks to tumor markers [J]. Trends Biotechnol 2001, 19:S40-8.
  • 4Natsume T, Yamauchi Y, Nakayama H, et al. A direct nanoflow liquid chromatography-tandem mass spectrometry system for interaction proteomics [J].Anal Chem 2002; 74:4725-33.
  • 5O'Farrell PH. High resolution two-dimensional electrophoresis of proteins [J]. J Bio Chem 1975;250:4007-21.
  • 6Cordwell S J, Basseal D J, Bjellqvist B, et al.Characterisation of basic protein from spiroplasma melliferum using novel immobilised pH gradients [J].Electrophoresis 1997; 18:1393-8.
  • 7Aebersold R, Mann M. Mass spectrometry-based proteomics [J]. Nature 2003: 422:198-207.
  • 8Gevaert K, Vandekerckhove J. Protein identification methods in proteomics [J]. Electrophoresis 2002;21:1145-54.
  • 9Gygi SP, Aebersold R. Mass spectrometry and proteomics[J]. Curr Opin Chem Biol 2000; 4:489-94.
  • 10Boguski MS, Mclntosh MW. Biomedical informatics for proteomics [J]. Nature 2003; 422:233-7.

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