成肌调节因子MyoD和myogenin在不同月龄DMD模型鼠mdx小鼠的表达

Expression of Myogenic Regulatory Factors MyoD and Myogenin in DMD Mouse Model mdx Mice at Different Age

目的探讨成肌调节因子MyoD和myogenin在不同月龄DMD模型鼠mdx鼠的表达情况。方法取不同月龄DMD模型鼠mdx鼠以及相应的同龄正常C57鼠的腓肠肌,冰冻切片后用HE染色显示肌肉病理,SABC-DAB染色检测成肌调节因子MyoD和myogenin的表达。结果不同月龄mdx鼠肌肉坏死和再生程度不同,MyoD和myogenin在1月龄mdx鼠表达最强,在13月龄mdx鼠仍有表达,在正常同龄C57鼠不表达。结论MyoD与Myogenin在肌肉损伤后的再生修复过程中起作用,可作为鉴定肌肉前体细胞和反映肌肉再生的指标。

Objective To explore the expression of myogenic regulatory factors MyoD and myogenin in DMD mouse model mdx mice at different age. Methods The gastrocnemius muscles of mdx and normal C57 mice at different age were collected and cut into frozen sections and stained by H.E. Pathologic changes of the muscles were examined by light microscopy. The expression of myogenic regulatory factors MyoD and myogenin were detected by SABC-DAB staining. Results The degree of muscle degeneration and regeneration was different in mdx mice at different ages. The expression of MyoD and myogenin was strongest in 1 month old mdx mice. The expression of MyoD and myogenin could be ever detected in 13 months old mdx mice and eotddn＇t be detected in normal C57 mice at the same age. Conclusions MyoD and myogenin play a role in muscle regeneration after muscle injury. They can be used as an index to identify muscle precursor cells and reflect muscle regeneration.