Wistar大鼠实验性变态反应性脑脊髓炎病理探讨

Pathological Changes of Relapsing-Remitting Experimental Allergic Encephalomyelitisin Wistar Rats

目的研究缓解-复发型EAE大鼠的基本病理改变。方法对缓解-复发型EAE大鼠进行HE染色、Weil髓鞘染色和改良的Bielschowsky染色,以免疫组化方法标记GFAP和MMP-2、MMP-9。结果组织内可见多个血管周围淋巴细胞浸润呈袖套样分布,伴有大片状脱髓鞘,部分脱失的髓鞘内有炎细胞浸润,轴索呈空泡样缺失,GFAP染色证明在旧病灶的周围部分可见星形胶质细胞增生,MMP-2、MMP-9在血管内皮细胞、细胞外基质、炎细胞及脑(脊)膜内呈阳性表达。结论①缓解-复发型EAE大鼠的组织病理学改变与临床表现一致,有活动性和非活动性病灶并存的现象。②MMP-2、MMP-9在活动性病灶内表达,参与疾病的发生。③缓解-复发型EAE病理改变与MS相似,是理想的动物模型。

Objective To study the pathological changes of relapsing-remitting experimental allergic encephalomyelitis （EAE） in Wistar rat. Method Tissue sections were processed with HE staining, Weil myelin staining and modified Bielschowsky staining. The expression and distribution of MMP-2 and -9 were detected with the method of IHC. Result There were many perivascular cuffings in tissues accompanied by large pieces of demyelination and inflammatory cells infiltrating into partial demyelinated tissues, axons with some vesicular loss, strong pastive GFAP around the old lesion, and MMP-2 and -9 were expressed in vascular endothelial cells, meninges, ECM and inflammatory cells. Conclusion （1） Pathological changes of relapsing-remltting EAE in rat were in coincidence with the clinical manifestations of patients and there were active and inactive lesions in the tissues. （2） MMP-2 and -9 were expressed in the active lesions and they participated in the pathogenesis. （3） Pathological changes of relapsing-remitting EAE were similar to multiple sclerosis and such rats are an ideal animal model.