摘要
Tong-de area of Qinghai Province had been identified by seroepidemiology as the high infection area of hepatitis B virus where the Zang population inhabit.To formulate a preventive strategy against hepatitis,we investigated in this area the biological characteristics and the genotypes of hepatitis B virus(HBV).PCR was used to amplify HBV S and C genes of samples from Zang population. The amplified products were sequenced,the nucleotide identities were analysed(MEGA 3 software) and the phylogenetic tree was constructed.The results showed that S gene of most of the HBV serum samples clustered into genotype D while C gene of the samples clustered into genotype C which demonstrated that HBV genotypes mostly were recombinants of HBV C and D genes in Zang population in Tong-de area of Qinghai Province.
Tong-de area of Qinghai Province had been identified by seroepidemiology as the high infection area of hepatitis B virus where the Zang population inhabit. To formulate a preventive strategy against hepatitis, we investigated in this area the biological characteristics and the genotypes of hepatitis B virus （HBV）. PCR was used to amplify HBV S and C genes of samples from Zang population. The amplified products were sequenced, the nucleotide identities were analysed （MEGA 3 software） and the phylogenetic tree was constructed. The results showed that S gene of most of the HBV serum samples clustered into genotype D while C gene of the samples clustered into genotype C which demonstrated that HBV genotypes mostly were recombinants of HBV C and D genes in Zang population in Tong-de area of Qinghai Province.
出处
《病毒学报》
CAS
CSCD
北大核心
2006年第5期400-402,共3页
Chinese Journal of Virology
