二硝基氯苯的结合不影响乙肝免疫球蛋白的抗体功能

Antibody titer of hepatitis B immune globulin remained intact after combined with ne

目的了解二硝基氯苯（DNCB）结合乙肝免疫球蛋白（HBIG）后对其抗体功能的影响。方法把HBIG（13mg／500μL PBS）分别与不同剂量（0．025，0．25，2．5mg）的DNCB（预先溶于DMSO，10mg／mL）结合，并予系列倍比稀释十次后，分别以DMSO及无菌水为两对照组，共组成五组，55个标本。以ELISA法分别检测乙肝表面抗体的效价。结果DMSO、无菌水、低剂量、中剂量与高剂量组五组的抗体阳性率分别为90．91％（10／11）、72．73％（8／11）、81．82％（9／11）、81．82％（9／11）和72．73％（8／11），其抗体阳性率以，检验无差异（P〉0．05）。经双因素方差分析（two-way analysis of variance），五组间在各稀释度的吸光值（OD450）上无差异（P〉0．05），而各稀释度处理组间则有非常显著差异（P〈0．01）。结论在一定范围内，DNCB的结合对HBIG的抗体效价没有影响，推测免疫球蛋白（抗体或单克隆抗体）可作为DNCB（或其它类似半抗原）靶向治疗的一种有效载体。

Objective To investigate the influence of dinitrochlorobenzene （DNCB） on the antibody titer of hepatitis B immune globulin （HBIG）. Methods The different doses （0.025, 0.25, and 2.5mg） of DNCB （dissolved in DMSO 10mg/mL） were combined with 13mg HBIG respectively, in a volume of 500ul phosphate buffer saline （PBS）. DMSO and pure water were used as 2 controls respectively. All the 5 groups were half-diluted consecutively with PBS and tested the antibody titer of HBIG with ELISA. Results The positive rates of 5 groups were 90.91% （10/11, DMSO group）, 72.73% （8/11, water group）, 81.82% （9/11, low dose group）, 81.82% （9/11, middle dose group）, and 72.73% （8/11, high dose group）, respectively. There were no differences in the 5 groups in terms of positive rate （P〉0.05）, nor the absorbance values （OD450） of HBIG in same dilution grade of any group with two-way anova （analysis of variance） （P〉0.05）. However, there is obvious differences between different dilution grades in any group with two-way anova （P〈0.01）. Conclusions It seems that DNCB has no effect on the antibody titer of HBIG at least in a certain concentration range, and the immune globulin （antibody） might be used as a carrier for DNCB （or other analog hapten） target therapy.