一氧化氮合酶与鼻咽癌血管形成及患者病程的关系

Expression of Inducible Nitric Oxide Synthase in Nasopharyngeal Carcinoma and Their Relationship with Angiogenesis and Its Clinical Significance

【目的】探讨诱生型一氧化氮合酶(iNOS)、血管内皮生长因子在鼻咽癌组织中的表达及微血管密度与患者病程的关系。【方法】用免疫组化方法检测77例鼻咽癌患者活检石蜡包埋标本的诱生型一氧化氮合成酶、血管内皮生长因子的表达,用Ⅷ因子相关抗原(F8)显示血管内皮细胞,根据F8阳性的血管内皮细胞计数测定肿瘤微血管密度。【结果】诱生型一氧化氮合成酶、血管内皮生长因子在鼻咽癌组织中的阳性表达率分别为34%(26/77)、32%(25/77),诱生型一氧化氮合成酶表达与血管内皮生长因子表达、微血管密度、T/N分期均呈正相关(P<0.05)。【结论】诱生型一氧化氮合成酶在鼻咽癌血管形成及肿瘤浸润与转移中起到促进作用。

[Objective] To investigate the expression of inducible nitric oxide synthase （iNOS） in nasopharygeal carcinoma and their relationship with angiogenesis and its clinical significance. [ Methods ] The tissue sections from 77 specimens of nasopharygeal carcinoma were examined immunohistochemically for the protein expression of iNOS, Factor Ⅷ related Antigen（F^8）, and vascular endothelial growth factor（VEGF）. [Results] The positive rate of iNOS and VEGF were detected in 34%（26/77） and 32%（25/77）respectively. There was a significant correlation among the expression of iNOS and the expression of VEGF and MVD （P 〈 0.05） and T/N stages.[Conclusion] The positive expression of inducible NOS （iNOS） in nasopharygeal carcinoma may contribute to angiogenesis, infdtrating and metastasis of tumor.