应用干扰素进行膀胱癌治疗的实验与临床观察

Experimantal and clinical research of Interferon on bladder cancer cell line T24 and patients

目的:探讨不同浓度的干扰素-α(IFN-α)对人膀胱癌细胞株T24的作用及25例膀胱癌瘤内注射的作用。方法:以MTT法测定不同浓度的IFN-α对T24细胞的杀伤作用,以DNA末端标记法(TUNEL法)、流式细胞术及电镜观察测定IFN-α对T24细胞的诱导凋亡作用;膀胱镜下瘤内注射IFN-α治疗25例膀胱癌患者。结果:低浓度IFN-α对T24细胞无明显杀伤作用,高浓度IFN-α(10000U/mL)可显著杀伤T24细胞,其作用有时间依赖性;高浓度的IFN-α可诱导T24细胞凋亡,F=84·544,P=0·007;术前肿瘤体积平均为(5·98±1·69)cm3,术中肿瘤体积平均为(2·31±0·77)cm3。与术前比较,注射IFN-α后患者肿瘤体积明显变小,差异有统计学意义,F=83·257,P=0·009。结论:高浓度IFN-α可直接杀伤膀胱癌细胞,诱导凋亡,有时间与剂量依赖性;局部应用IFN-α可能成为膀胱癌综合治疗的有效手段之一。

OBJECTIVE： To investigate the effects of different concentrations of interferon-α （IFN α） on human bladder cancer cell line T24 and on 25 patiams with bladder cancer. METHODS： The cytotoxicity effect of IFN-α was as sayed by MTT. Apoptosis was measured by flow cytometry, TUNEL and electron microscopy. Twenty-five cases were treated with local injection of IFN-α before operation. RESULTS： Low dose IFN-α did not have any significant effects on growth of T24 cells. High dose IFN-α （10 000 U/mL） induced apoptosis and had significant cytotoxicity effects with dependence of time and dosage on T24 cells （F= 84. 544, P = 0. 007）. Tumor size became small obviously after the injection （F= 83. 257, P= 0. 009）. CONCLUSIONS： High-dose IFN-α （10 000 U/mL） has significant cytotoxicity effects on T24 cells and induces apoptosis of bladder cancer cells. Local injection of IFN-α may become one of effective therapy for bladder cancer.