射干麻黄煎剂影响哮喘豚鼠外周血浆白细胞介素5,10的变化

Effect of shegan mahuang decoction on the changes of interleukin 5 and interleukin 10 in peripheral plasm of asthma guinea pigs

目的:观察射干麻黄汤对哮喘豚鼠外周血浆促炎细胞因子白细胞介素5、抑炎细胞因子白细胞介素10生理性平衡的影响。方法:实验于2005-09/2006-02在广东药学院动物实验室完成。选取健康Hartley种雄性豚鼠70只,随机数字表法分成7组:正常对照组、哮喘模型对照组、激素治疗组、氨茶碱治疗组、中药0.16g/mL浓度组、中药0.32g/mL浓度组、中药0.64g/mL浓度组,10只/组。除正常对照组外,其余各组均建立哮喘动物模型。建模第22天,于每次卵蛋白溶液激发后1h灌胃给药,1次/d,连续7d,给药量均为23.25mL/kg。①正常对照组、哮喘模型对照组给予生理盐水治疗。②激素治疗组精确称取醋酸强的松片33.33mg,溶于100mL生理盐水中。③氨茶碱治疗组精确称取氨茶碱片100mg,溶于100mL生理盐水中。④中药配方:射干9g,麻黄12g,半夏12g,紫菀9g,款冬花9g,大枣12g,细辛9g,生姜12g,五味子12g。中药0.16g/mL浓度组生药用量为上述配方的1/2,中药0.32g/mL浓度组生药用量与上述配方相同,中药0.64g/mL浓度组生药用量为上述配方的1倍。各组生药分开煎制,按传统煎药方法制300mL中药煎剂,每毫升溶液含生药量分别为0.16g,0.32g,0.64g。⑤分别于建模第21,28天心脏穿刺取血,离心收集上清液,ELISA法检测各组豚鼠外周血白细胞介素5、白细胞介素10的浓度。结果:实验选取70只豚鼠均进入结果分析。治疗前后各组外周血白细胞介素5、白细胞介素10浓度的变化:①正常对照组、哮喘模型对照组外周血白细胞介素5、白细胞介素10的浓度治疗前后均基本相似(P>0.05)。②激素治疗组、氨茶碱治疗组治疗后白细胞介素5浓度均明显低于治疗前[(130.21±19.43),(507.14±56.17);(173.69±28.97),(507.60±51.29)ng/L;P均<0.05];但白细胞介素10浓度均与正常对照组基本相似(P>0.05)。③中药0.16,0.32,0.64g/mL浓度组治疗后白细胞介素5浓度均低于治疗前[(265.61±42.55),(505.83±33.62);(193.34±29.84),(506.66±51.29);(166.30±36.44),(505.91±66.16)ng/L;P均<0.05],但明显高于正常对照组(P<0.05或0.01);白细胞介素10浓度均高于治疗前(P<0.05或0.01),但明显低于正常对照组、激素治疗组、氨茶碱治疗组(P<0.05或0.01)。结论:中药射干麻黄汤能改善促炎/抑炎细胞因子失衡,从而起到抑制炎症、缓解气道高反应性的作用。

AIM： To observe the effects ofshegan mahuang decoction on the physiologic balance of pro-inflammatory cytokine interleukin 5 （IL-5） and anti-inflammatory cytokine IL-10 in the peripheral plasma of asthma guinea pigs. METHODS： The experiment was conducted in the Experimental Animal Laboratory of Guangdong Pharmaceutical College from September 2005 to February 2006. Seventy healthy Hartley male guinea pigs were randomized into 7 groups： control group, model group, hormone group, aminophylline group, and three herbs group at different concentrations （0.16 g/mL, 0,32 g/mL and 0.64 g/ml,）, with 10 guinea pigs in each group. Except normal control group, the guinea pigs of other groups were adopted to induce asthma models. On the 22^nd day of modeling, the guinea pigs were given intragastric injeetion one hour after every egg albumen excitation, once daily for continuous 7 days at the dose of 23.25 mL/kg. ①Control group and model group were treated with saline,② Hormone group was treated with prednisone acetate tablets at exact dose of 33.33 mg dissolving in 100 mL saline.③Aminophylline group was given 100 mg aminophylline tablets dissolving in 100 mL saline.④Herbs recipe： Blackberrylily rhizome, tatarian aster root, common colts foot flower and siebold wildginger herb of 9 g in each, and ephedra, pinellia tuber, jujube, fresh ginger and Chinese magnoliavine fruit of 12 g in each. The crude drug in the 0.16 g/mL concentration group was half of above recipe, and those in 0.32 g/mL group was the same as above recipe while those in 0.64 g/mL group was one time more than above recipe. Herbs preparations of 300 mL were decocted traditionally in the corresponding groups, including the crude drug of 0.16 g, 0.32 g and 0.64 g, respectively. ⑤On the 22^nd and 28^th days of modeling, blood sample was collected via heart puncture, and supernatant was obtained by centrifugation. ELISA method was used to detect the concentrations of IL-5 and IL-10 in the peripheral blood of guinea pigs. RESULTS： Totally 70 guinea pigs were involved in the result analysis. Changes of the concentrations of IL-5 and IL-10 before and after treatment： ①The concentrations of IL-5 and IL-10 were not changed in control group and model group after treatment （P 〉 0.05）.②Compared with before treatment, the concentration of IL-5 after treatment was obviously decreased in hormone group and aminophylline group [（130.21±19.43）, （507.14±56.17）; （173.69±28.97）, （507.60±51.29） ng/L; P 〈 0.05], but the concentration of IL-10 was similar with that of control group （P 〉 0.05）, ③Compared with before treatment, the concentrations of IL-5 were decreased in herbs groups after treatment [（265.61±42.55）, （505.83±33.62）; （193.34±29.84）, （506.66±51.29）; （166.30±36.44）, （505.91±66.16） ng/L; P 〈 0.05], which were obvious higher than those of control group （P 〈 0.05 or 0.01）. The concentrations of IL-10 in herbs groups were higher than those of before treatment （P 〈 0.05 or 0.01）, but still lower than those of control group, hormone group and aminophylline group （P 〈 0.05 or 0.01）. CONCLUSION： Shegan mahuang decoction can inhibit the inflammation and relieve the airway hyperreactivity by improving the unbalance of pro- and anti-inflammatory cytokines.