摘要
目的:观察射干麻黄汤对哮喘豚鼠外周血浆促炎细胞因子白细胞介素5、抑炎细胞因子白细胞介素10生理性平衡的影响。方法:实验于2005-09/2006-02在广东药学院动物实验室完成。选取健康Hartley种雄性豚鼠70只,随机数字表法分成7组:正常对照组、哮喘模型对照组、激素治疗组、氨茶碱治疗组、中药0.16g/mL浓度组、中药0.32g/mL浓度组、中药0.64g/mL浓度组,10只/组。除正常对照组外,其余各组均建立哮喘动物模型。建模第22天,于每次卵蛋白溶液激发后1h灌胃给药,1次/d,连续7d,给药量均为23.25mL/kg。①正常对照组、哮喘模型对照组给予生理盐水治疗。②激素治疗组精确称取醋酸强的松片33.33mg,溶于100mL生理盐水中。③氨茶碱治疗组精确称取氨茶碱片100mg,溶于100mL生理盐水中。④中药配方:射干9g,麻黄12g,半夏12g,紫菀9g,款冬花9g,大枣12g,细辛9g,生姜12g,五味子12g。中药0.16g/mL浓度组生药用量为上述配方的1/2,中药0.32g/mL浓度组生药用量与上述配方相同,中药0.64g/mL浓度组生药用量为上述配方的1倍。各组生药分开煎制,按传统煎药方法制300mL中药煎剂,每毫升溶液含生药量分别为0.16g,0.32g,0.64g。⑤分别于建模第21,28天心脏穿刺取血,离心收集上清液,ELISA法检测各组豚鼠外周血白细胞介素5、白细胞介素10的浓度。结果:实验选取70只豚鼠均进入结果分析。治疗前后各组外周血白细胞介素5、白细胞介素10浓度的变化:①正常对照组、哮喘模型对照组外周血白细胞介素5、白细胞介素10的浓度治疗前后均基本相似(P>0.05)。②激素治疗组、氨茶碱治疗组治疗后白细胞介素5浓度均明显低于治疗前[(130.21±19.43),(507.14±56.17);(173.69±28.97),(507.60±51.29)ng/L;P均<0.05];但白细胞介素10浓度均与正常对照组基本相似(P>0.05)。③中药0.16,0.32,0.64g/mL浓度组治疗后白细胞介素5浓度均低于治疗前[(265.61±42.55),(505.83±33.62);(193.34±29.84),(506.66±51.29);(166.30±36.44),(505.91±66.16)ng/L;P均<0.05],但明显高于正常对照组(P<0.05或0.01);白细胞介素10浓度均高于治疗前(P<0.05或0.01),但明显低于正常对照组、激素治疗组、氨茶碱治疗组(P<0.05或0.01)。结论:中药射干麻黄汤能改善促炎/抑炎细胞因子失衡,从而起到抑制炎症、缓解气道高反应性的作用。
AIM: To observe the effects ofshegan mahuang decoction on the physiologic balance of pro-inflammatory cytokine interleukin 5 (IL-5) and anti-inflammatory cytokine IL-10 in the peripheral plasma of asthma guinea pigs. METHODS: The experiment was conducted in the Experimental Animal Laboratory of Guangdong Pharmaceutical College from September 2005 to February 2006. Seventy healthy Hartley male guinea pigs were randomized into 7 groups: control group, model group, hormone group, aminophylline group, and three herbs group at different concentrations (0.16 g/mL, 0,32 g/mL and 0.64 g/ml,), with 10 guinea pigs in each group. Except normal control group, the guinea pigs of other groups were adopted to induce asthma models. On the 22^nd day of modeling, the guinea pigs were given intragastric injeetion one hour after every egg albumen excitation, once daily for continuous 7 days at the dose of 23.25 mL/kg. ①Control group and model group were treated with saline,② Hormone group was treated with prednisone acetate tablets at exact dose of 33.33 mg dissolving in 100 mL saline.③Aminophylline group was given 100 mg aminophylline tablets dissolving in 100 mL saline.④Herbs recipe: Blackberrylily rhizome, tatarian aster root, common colts foot flower and siebold wildginger herb of 9 g in each, and ephedra, pinellia tuber, jujube, fresh ginger and Chinese magnoliavine fruit of 12 g in each. The crude drug in the 0.16 g/mL concentration group was half of above recipe, and those in 0.32 g/mL group was the same as above recipe while those in 0.64 g/mL group was one time more than above recipe. Herbs preparations of 300 mL were decocted traditionally in the corresponding groups, including the crude drug of 0.16 g, 0.32 g and 0.64 g, respectively. ⑤On the 22^nd and 28^th days of modeling, blood sample was collected via heart puncture, and supernatant was obtained by centrifugation. ELISA method was used to detect the concentrations of IL-5 and IL-10 in the peripheral blood of guinea pigs. RESULTS: Totally 70 guinea pigs were involved in the result analysis. Changes of the concentrations of IL-5 and IL-10 before and after treatment: ①The concentrations of IL-5 and IL-10 were not changed in control group and model group after treatment (P 〉 0.05).②Compared with before treatment, the concentration of IL-5 after treatment was obviously decreased in hormone group and aminophylline group [(130.21±19.43), (507.14±56.17); (173.69±28.97), (507.60±51.29) ng/L; P 〈 0.05], but the concentration of IL-10 was similar with that of control group (P 〉 0.05), ③Compared with before treatment, the concentrations of IL-5 were decreased in herbs groups after treatment [(265.61±42.55), (505.83±33.62); (193.34±29.84), (506.66±51.29); (166.30±36.44), (505.91±66.16) ng/L; P 〈 0.05], which were obvious higher than those of control group (P 〈 0.05 or 0.01). The concentrations of IL-10 in herbs groups were higher than those of before treatment (P 〈 0.05 or 0.01), but still lower than those of control group, hormone group and aminophylline group (P 〈 0.05 or 0.01). CONCLUSION: Shegan mahuang decoction can inhibit the inflammation and relieve the airway hyperreactivity by improving the unbalance of pro- and anti-inflammatory cytokines.
出处
《中国临床康复》
CSCD
北大核心
2006年第35期63-65,共3页
Chinese Journal of Clinical Rehabilitation