糖尿病大鼠肾脏抗氧化酶变化与灯盏花素的干预效应

Interventional effects of breviscapine on renal antioxidant enzymes in diabetic rats

目的:在链脲佐菌素诱导的糖尿病大鼠模型上,探讨灯盏花素对糖尿病大鼠肾脏氧化应激反应的影响。方法:实验于2004-04/2005-04在武汉大学医学院实验中心完成。健康Wistar大鼠30只,随机分为正常组、糖尿病对照组和灯盏花素治疗组,糖尿病对照组和灯盏花素治疗组采用一次性尾静脉注射链脲佐菌素60mg/kg,正常组则用等量柠檬酸缓冲液尾静脉注射,72h后,尾静脉采血测血糖≥16.7mmol/L,确定造模均成功。灯盏花素治疗组灯盏花素20mg/(kg·d)灌胃给药;正常组、糖尿病对照组给予等量生理盐水灌胃。实验期间自由进水、进食,不使用胰岛素及其他降糖药物。12周后,测定各组空腹血糖、血总胆固醇、三酰甘油、尿素氮、肌酐、糖化血红蛋白水平,免疫比浊法测尿蛋白排泄量,运用比色法测定肾脏皮质丙二醛含量、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性。结果:①糖尿病对照组肾脏超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性显著低于正常组眼(128.17±9.33),(58.67±4.67),(106.5±13.0)μkat/g;(215.83±49.50),(98.17±7.50),(178.0±17.0)μkat/g,P<0.05演。②灯盏花素治疗组肾脏超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性显著高于糖尿病对照组眼(170.83±26.33),(77.83±6.50),(139.5±15.0)μkat/g;(128.17±9.33),(58.67±4.67),(106.5±13.0)μkat/g,P<0.05演。③糖尿病对照组肾脏丙二醛含量显著高于正常组,而灯盏花素治疗组肾脏丙二醛含量显著低于糖尿病对照组眼(13.57±3.89),(3.62±0.18)μmol/g;(6.87±1.96),(13.57±3.89)μmol/g,P<0.05演。结论:灯盏花素可通过保护肾脏抗氧化酶,抑制氧化应激反应,对糖尿病大鼠肾脏产生保护作用。

AIM： To investigate the effects of breviscapine on oxidative stress of kidney in streptozotocin-induced diabetic rats. METHODS： The experiment was conducted in the Experimental Center of Medical College of Wuhan University from April 2004 to April 2005. Thirty healthy Wistar rats were randomly divided into normal group, diabetic control group and breviscapine treatment group. Rats in the diabetic control group and breviscapine treatment group were injected with 60 mg/kg breviscapine via the tail vein, while rats in the normal control group were injected with citrate buffer solution at the same volume. 72 hours after that, blood was obtained from the tail vein to measure the blood glucose. Rats with the blood glucose ≥16.7 mmol/L were taken as successful modeling. Rats in the breviscapine treatment group were intragstrically administrated with 20 mg/kg breviscapine per day. Rats in the normal group and diabetic control group were given gastric perfusion of normal saline at the same volume. Rats ate and drank freely in the experiment without administrated with insulin or phenformin. 12 weeks after that, the blood glucose, blood total cholesterol, triglyceride, urea nitrogen, creatinine and glycosylated hemoglobin were determined. The excretion of urinary protein was measured by immune turbidimetry. The activities of malondialdehyde, superoxide dismutase, peroxidase and glutathione peroxidase in renal cortex were measured by chromatometry. RESULTS： ①The activities of superoxide dismutase, hydrogen peroxidase and glutathione peroxidase in rats of the diabetic control group were significantly lower than those in the normal group [（128.17±9.33）, （58.67 ±4.67）,（106.5±13.0） μkat/g;（215.83±49.50）,（98.17±7.50）,（178.0±17.0） μkat/g, P 〈 0.05]. ②The activities of superoxide dismutase,hydrogen peroxidase and glutathione peroxidase in rats of the breviseapine treatment group were remarkably higher than those in the diabetic control group[（170.83±26.33）, （77.83 ±6.50）, （139.5 ±15.0） μkat/g; （128.17 ±9.33）, （58.67 ±4.67）, （106.5 ±13.0） μkat/g,P 〈 0.05]. ③The malondialdehyde content was significantly higher in the diabetic control group than in normal group, while it was remarkably lower in the breviscapine treatment group than the diabetic control group [（13.57±3.89）, （3.62±0.18） μmol/g; （6.87±1.96）, （13.57±3.89） μmol/g,P 〈 0.05]. CONCLUSION： Breviscapine can protect the kidney of rats by protecting antioxidant enzymes and suppress the oxidative stress in kidney of diabetic rats.