加味保元汤对束缚应激小鼠细胞免疫功能的影响

Influence of modified Baoyuantang on cellular immune function of restrained stress mice

目的:对束缚应激小鼠给予加味保元汤灌胃后,检测T淋巴细胞转化率,观察加味保元汤对细胞免疫功能的影响。方法:实验于2002-07/10在大连大学医学院生理学实验室完成。健康、雄性、昆明品系小白鼠100只,随机等分为5组:①正常组,只禁食水17h,不作任何其他处理。②应激组,束缚17h,束缚期间禁食水。③正常加药组,不束缚但与应激加药组相同时间灌胃给加味保元汤水煎剂(人参250g,黄芪300g,肉桂250g,甘草150g,五味子250g,熬制成水煎剂)。④应激加药组,束缚17h,束缚前2d,每天上、下午各给药1次,束缚前1,3,5h及束缚后5,7,9h分别再给药1次。⑤应激加水组,束缚17h,束缚前2天,每天上、下午各给水1次,束缚前1,3,5h及束缚后5,7,9h分别再给水1次,其容积与加味保元汤水煎剂相同。各组动物经各实验因素处理后,断髓处死,取脾,去筋膜,检测T淋巴细胞转化率。T淋巴细胞转化率=转化的淋巴细胞数/(转化的淋巴细胞数+未转化的淋巴细胞数)×100%。结果:实验小鼠100只进行T淋巴细胞转化率检测。加水对束缚应激没有影响;在加味保元汤作用下,使束缚应激致免疫功能低下基本恢复正常;加味保元汤可以提高正常小鼠的T淋巴细胞转化率,并可以使束缚应激后降低的T淋巴细胞转化率基本恢复正常。束缚应激加药组T淋巴细胞转化率明显高于束缚应激组(应激组为28.30%,应激加水组为21.90%,正常组为38.00%,应激加药组为37.20%,正常加药组为51.70%,P<0.005)。结论:加味保元汤可提高束缚应激小鼠细胞免疫功能。

AIM： To observe the effect of modified Baoyuantang （BYT） on cellular immune function after detecting transformation rate of T lymphocyte in restrained stress mice after being treated with gastric perfusion with BYT. METHODS： The experiment was performed at the Laboratory of Physiology, Medical College, Dalian University from July to October 2002. 100 healthy male Kunming white mice were randomly divided into 5 groups： ① normal group, fasting water for 17 hours, without any other disposal,② stress group, restraining for 17 hours and fasting water during constraint,③normal drug group, no constraint, treated with gastric perfusion with BYT （250 g radix ginseng, 300 g milkvetch root, 250 g cinnamon, 150 g liquorice root, 250 g fructus schizandrae, decocting） at the same time as stress plus drug group, ④stress plus drug group, restraining for 17 hours, administration once in the morning and afternoon every day for 2 days before restraining; administration once at hours 1, 3 and 5 before restraining and hours 5, 7 and 9 after restraining, ⑤stress plus water group, restraining for 17 hours, being treated with water once in the morning and afternoon every day for 2 days before restraining; Giving water once at hours 1, 3 and 5 before restraining and hours 5, 7 and 9 after restraining, and its volume was the same as that of the modified BYT. After being disposed by each empirical factor, the white mice in each group were killed by breaking marrow so as to take out spleen, and anadesma was removed. Transformation rate of T lymphocyte was measured. Transformation rate of T lymphocyte was equal to number of transformed lymphocyte/（number of transformed lymphocyte＋number of non-transformed） ×100%. RESULTS： Totally 100 experimental mice were examined in transformation rate of T lymphocyte. Water had no effect on restrained stress. Low immunologic function induced by retrained stress recovered to normal basically after being treated with modified BYT. The BYT could elevate the transformation rate of T lymphocyte in normal mice, and make decreased transformation rate of T lymphocyte recover to normal after restrained stress. Transformation rate of T lymphocyte in the restrained stress plus drug group was higher obviously than that in the restrained stress group （28.30% in the stress group, 21.90% in the stress plus water group, 38.00% in the normal group, 37.20% in the stress plus drug group, 51.70% in the normal drug group, P 〈 0.005）. CONCLUSION： Modified BYT may increase the cellular immune function in restrained stress mice.