DNA与对氨基苯酚相互作用的初步研究

Study on the interaction of DNA and p-aminophenol

目的:探讨DNA与对氨基苯酚的相互作用。方法:在0.05 mol/L Na2HPO4-0.05 mol/L NaH2PO4-0.10mol/L NaC l缓冲体系(pH=7.30)中研究对氨基苯酚在核苷三磷酸修饰电极及DNA修饰电极上的电化学行为。结果:双链DNA(dsDNA)和单链DNA(ssDNA)均可减小对氨基苯酚的氧化峰峰电流,在三磷酸腺苷(dATP)、三磷酸鸟苷(dGTP)修饰电极上的电化学行为与之相似。结论:三磷酸腺苷(dATP)、三磷酸鸟苷(dGTP)和DNA与对亚氨基苯醌以较强的氢键结合,与对氨基苯酚作用力较小。

Objective： To study on the interaction between p-aminophenol and DNA. Methods： The electrochemical behavior of p-aminophenol at nucleoside triphosphate, single chain DNA （ssDNA） and double chain DNA （dsDNA） modified electrode in 0.05mol/L Na2HPO4 -0.05 mol/L NaH2PO4 -0. 10mol/L NaCl buffer solution （ pH = 7.30） were studied in this paper. Results： The dATP and dGTP could decrease the current of p-aminophenol and shift slightly its potential; the similar behavior at the dsDNA and ssDNA modified electrode was found. Conclusion： The interaction between p-quinonimine and DNA ascribed to hydrogen bonding, however, this force between . DNA and p-aminophenol was smaller than that of p-quinonimine and DNA