中药黄芩苷对脂多糖刺激人牙周膜成纤维细胞分泌肿瘤坏死因子-α的影响

Effects of bacailin on TNF-αproduction of human periodontal ligament fibroblasts by stimulation of LPS

目的研究中药黄芩苷对脂多糖(LPS)刺激人牙周膜成纤维细胞(HPLFs)分泌肿瘤坏死因子-α(TNF-α)的影响。方法采用原代培养HPLFs技术和酶联免疫检测方法(ELISA),检测培养上清液中TNF-α的水平。结果0．001～10μg／ml黄芩苷和2μg／ml地塞米松均可显著抑制LPS刺激HPLFs分泌TNF-α的活性(P＜0．01),而不同浓度组之间比较,抑制作用无显著性差异(P＞0．05)。结论黄芩苷对LPS刺激HPLFs合成和分泌TNF-α有显著抑制作用,与地塞米松抗炎效果相近,作用机制与抑制TNF-α等炎性细胞因子过度产生有关,揭示了黄芩苷可能的抗炎作用机制。

Objective To study the effects of the Chinese baicalin on human periodontal ligament fibroblasts （HPLFs） secreting tumor necrosis factor alpha （TNF-α） stimulated by lipopolysaccharide （LPS） of Eseherichia coil （E. coil）. Methods The cell culture technique in vitro, Enzyme- linked immunosorbent assay （ELISA） were used to observe the effects of baicalin on the level of TNF -α in culture supernatant. Results Baicalin and dexamethasone could significantly inhibit the activity of TNF-α induced by LPS and significantly suppressed TNF -α production of HPLFs stimulated with LPS （ P 〈 0.01 ）, but among the different baicalin concentration groups and dexamethasone group, there is no significant difference （ P 〉 0.05 ）. Conclusion Human periodontal ligament fibroblasts have the ability of synthesizing and secreting TNF -α , and LPS is a potent stimulation for HPLFs to generate TNF -α ; Baicalin and dexamethasone can significantly inhibit the TNF -α production of HPLFs stimulated by LPS and there is not significantly difference between both.