Alzheimer氏病Aβ与Cu(II)相互作用的研究方法

Copper(Ⅱ) Interactions With Alzheimer Amyloid β-Peptides:Experimental Methods

大量老年斑沉积在脑神经细胞外是老年性痴呆的一个重要病理特征。现已证明老年斑的重要组成成分是一种小片段肽Aβ,另外,发现老年斑中也存在一些过渡态的金属离子如Zn(II)和Cu(II)等。近年的一些实验研究提示,Aβ与Cu(II)络合成复合物可能是Aβ神经毒性作用的机制之一。以A1β-40为例,对在体外条件下研究AβCu(Ⅱ)复合物性质及生物学活性的实验方法进行介绍,这将有利于对Aβ神经毒性作用机制更加深入和广泛的研究。

Alzheimer＇ s disease（AD） is characterized by innumerable deposits of extracellular senile plaques. A small peptide, amyloid β-peptide（Aβ）, plays a critical role in the initial build-up of these senile plaques and is the main constituent of the amyloid deposits in them. In addition, it is reported that metals such as Zn（Ⅱ） and Cu（Ⅱ） have been found in the senile plaques. There is now direct evidence that copper is bound to Aβ in senile plaque of AD. Copper is also linked with the neurotoxieity of Aβ and free radical damage. Taking Aβ1-40 as sample, discusses some experimental methods of copper（ Ⅱ ） interations with AD amyloid β-peptides to facilitate researches on disclosing the mechanism of neurotoxicity of Aβ.