醇沉对纯化金银花中绿原酸工艺的影响

Effect of ethanol precipitation on chlorogenic acid from Flos Lonicerae Japonica

目的:测定醇沉对绿原酸纯化工艺的影响。方法:采用HPLC测定方法对不同醇沉度提取金银花粗粉中绿原酸的效果进行了比较。结果:最佳醇沉度为60。采用醇沉、萃取复合工艺可将粗粉中绿原酸含量从5.5提高到37.72,收率为83.53。结论:醇沉可有效去除金银花粗粉中的杂质,且绿原酸收率高达90.16。

AIM： To test the effect of ethanol precipitation on the purifying technology of chlorogenic acid （CGA）. METHODS： Using HPLC, the effect of the extraction of CGA from the crude Flos Lonicerae Japonica powder with three concentrations of ethanol was compared. RESULTS ： Ethanol with concentration of 60% was optimal. CGA content was raised from 5.5% to 37.72% by use of ethanol precipitation and extraction in combination. CONCLUSION： The ethanol precipitation can get rid of the impurity in the crude powder of Flos Lonicerae Japonica effectively and the yield of CGA can reach 90.16%.