用正交设计优选四妙勇安汤方药的半仿生提取工艺条件

Orthogonal design for optimizing the semi-bionic extraction of Simiao Yongan Decoction

目的:以四妙勇安汤为例,探讨中药复方用半仿生提取(SBE)的研究方法。方法:以绿原酸、阿魏酸、甘草酸、总多糖、醇浸出物、干浸膏为指标成分,用正交试验优选半仿生提取的工艺条件。结果:优选出的工艺条件为:药材粉碎成过4目筛的粗粉,用20乙醇提取3次,3次提取的pH值依次为3.50、7.50、8.50;溶剂用量依次为10、8、8倍,提取时间依次为1.5、1.0、1.0h,结论:验证试验结果表明,优选出的SBE工艺条件稳定、可行,优于原SBE工艺条件。

AIM： To investigate the method for using semi-bionic extraction （SBE） on Simiao Yongan Decoction as an example. METHODS ： Orthogonal design was used to optimize the extraction conditions of SBE with the evaluation markers such as chlorogenic acid, ferulic acid, glycyrrhizic acid, total polysaccharide, ethanol soluble extractives and dried extract. RESULTS： The optimized SBE conditions were as follows： The medical material was powdered through a sieve with 4 meshes） and extracted with 20% ethanol for three times, the pH of the three extractions were in proper order 3.50, 7.50,8.50; the solvent volume was 10,8,8 times, respectively, and the extraction time was 1.5,1.0,1.0 h, respectively. CONCLUSION： The optimum extraction conditions are stable and practicable, and surpass the original SBE extraction conditions.