虎刺梅愈伤组织的诱导和快速繁殖

Tissue Culture and Rapid Propagation of Euphorbiamilli var.Splendens

以虎刺梅不同部位为试验材料,不同浓度6-BA、NAA进行愈伤组织的诱导和快速繁殖。结果表明,MS培养基附加6-BA 0.5 mg/L+NAA 1.0 mg/L的激素组合对花柄愈伤组织诱导效果最佳;将产生的愈伤组织团转入分化培养基中诱导不定芽,其最适分化培养基为MS附加6-BA 3.0 mg/L+NAA 0.2 mg/L;再生苗在附加6-BA 2.5 mg/L+NAA 0.5 mg/L的MS培养基上生根培养效果较好。

To explore the effective and rapid propagating method of Euphorbiamilli var. Splendens,the stem, flower stalk and leaf of Euphorbiamilli var. Splendens were selected as explants for callus induction and propagation. The experiment was carried out under the conditions of different concentration of 6-BA and IAA. It was showed that MS medium supplemented with the combination of 6-BA 0.5 mg/L and NAA 1.0 mg/L was the best to callus induction with section flower stalk as explants. The optimtan differentiation medium for regeneration of shoots was MS medium with 6-BA 3.0 mg/L and IAA 0.2 mg/L. The plantlets were rooted on MS medium with 6-BA 2.5 mg/L and IAA 0.5 mg/L with good results. Therefore both of stem and flower stalk can be used as explants. Since the quantity of section flower stalk was large and the materials were easily gathered, the inducting effect of callus was much better than stem, and flower stalk was also considered as an ideal explants for callus induction and propagation.