儿童急性白血病survivin基因表达及临床意义

Expression of survivin gene in children with acute leukemia

目的探讨儿童急性白血病(AL)survivin基因的表达及其临床意义。方法用逆转录多聚酶链式反应技术(RT-PCR),对42例AL患儿和13例完全缓解AL(AL-CR)患儿及15例非恶性疾病患儿骨髓进行survivin基因表达的检测。结果Survivin基因的表达在AL组明显高于对照组和完全缓解组(P<0.01),复发组高于初发组(P<0.01);survivin基因在AL的高危组和标危组中的表达有显著性差异(P<0.01);化疗后达完全缓解的患者survivin表达水平与治疗前及治疗后均明显低于未能缓解者(P均<0.05)。结论儿童AL的发生发展可能与survivin基因的过度表达密切相关,可作为化疗疗效的观察指标;survivin高表达者易复发,可作为估计预后的指标之一。

[Objective] To investigate the expression and significance of survivin gene in children with acute leukemia （AL）. [Methods] The expression of survivin gene were examined by reverse transcrlption-polymerase chain reaction （RT-PCR） in bone marrow cells from 42 cases of childhood acute leukemia, 13 cases of complete remission AL （AL-CR） and 15 cases of children without malignance disease in control group. [Results] The expression of survivin in AL group was higher than that of control group and complete remission group （P 〈0.01）. Between relapsed group and incipient group, the level of survivin mRNA expression was significantly different （P 〈0.01）. Among patients in incipient group, the level of survivin mRNA expression in high-risk patients was significantly higher than that in standard-risk patients （P 〈0.01）. We had an effect evaluation on 19 cases of AL patients. The survivin mRNA expression level in patients who achieved complete remission （CR） was significantly lower than that in patients with no remission （NR） both before and after chemotherapy （P 〈0.05）. [Conclusions] Survivin gene may contribute to the pathogenesis and the progress of the childhood acute leukemia; Survivin gene may become a marker for the treatment evaluation for children with acute leukemia. Survivin may be related to poor prognosis of childhood acute leukemia.