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水稻白叶枯病菌gacA基因的克隆、测序及敲除研究 被引量：1

Cloning,sequencing and knock-out of gacA gene from Xanthomonas oryzae pv.oryzae
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摘要 根据黄单胞菌gacA基因的同源性设计简并引物,采用PCR方法从水稻白叶枯病菌(Xanthom onas oryzaepv.oryzae,Xoo)中克隆了gacA同源基因,命名为gacAXoo。序列比较显示,该基因在黄单胞菌中是相对保守的。蛋白质保守结构域搜索表明,GacAXoo属于LuxR家庭的一员,具有与其他GacA蛋白相似的结构。通过同源重组的方法,构建了gacAXoo的插入突变体,为下一步研究gacAXoo的功能奠定了良好的基础。 A gacA homologue, designated gacAxoo, was cloned from the genomic DNA of Xanthornonas oryzae pv. oryzae （Xoo）, a bacterium that causes bacterial blight of rice, with degenerated primers by polymerase amplification reaction （PCR）. NCBI blast search indicated that GacAxoo had a similar structure to that of other GacA proteins, and had conserved domain of LuxR-like proteins. Sequence comparison showed that gacAxoo was conserved in the Xanthomonas genus. A gacAxoo disruption mutant was successfully generated by a single cross-over event, selected by Amp resistance on NA medium, and confirmed by PCR and Southern blot. This mutant is a useful tool for further study on the function of gacAxoo in X. oryzae pv. oryzae.
作者 杨万风 刘红霞 娄旸 胡白石 许志刚 刘凤权
机构地区 南京农业大学农业部病虫监测与治理重点开放实验室
出处 《南京农业大学学报》 CAS CSCD 北大核心 2006年第3期33-38,共6页 Journal of Nanjing Agricultural University
基金 国家自然科学基金项目(30070497) 国家高技术研究发展计划(2002AA245041)
关键词 水稻 白叶枯病菌 gacA基因 基因敲除 Xanthomonas oryzae pv. oryzae （ Xoo） gacA gene gene knock-out
分类号 S435.111 [农业科学—农业昆虫与害虫防治]
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参考文献14

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同被引文献22

  • 1高世强,张新建,吴茂森,何晨阳.水稻白叶枯病菌在寄主体内外不同生长条件下致病基因差异表达的分析[J].植物病理学报,2009,39(6):653-658. 被引量：1
  • 2杨万风,陈磊,刘红霞,胡白石,刘凤权.水稻条斑病菌gacA基因的克隆及功能初析[J].微生物学报,2007,47(2):208-212. 被引量：2
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南京农业大学学报
2006年 第3期

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